百草枯可抑制培养的人脐静脉内皮细胞中表达的血管紧张素转换酶的活性。

Paraquat depresses the activity of angiotensin converting enzyme expressed by human umbilical vein endothelial cells in culture.

作者信息

Watanabe K, Tamaru N, Yoshida M

机构信息

Second Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.

出版信息

Cell Biol Int Rep. 1991 Mar;15(3):205-10. doi: 10.1016/0309-1651(91)90152-9.

DOI:10.1016/0309-1651(91)90152-9

PMID:1851671

Abstract

The activity of angiotensin converting enzyme (ACE) in cell lysate of cultured human umbilical vein endothelial cells (HUVEC) after a 24-hour incubation with 10(-3) and 10(-4)M of paraquat (PQ) was decreased. However, LDH released into the culture medium of HUVEC during the 24-hour incubation with PQ was not increased. Many investigators show that the change in serum ACE activity reflects the impairment of vascular endothelial cells. We showed in this report that ACE was decreased even at an early stage of endothelial injury induced by PQ, when LDH release is not yet increased.

摘要

在用10⁻³和10⁻⁴M百草枯（PQ）孵育24小时后，培养的人脐静脉内皮细胞（HUVEC）细胞裂解物中血管紧张素转换酶（ACE）的活性降低。然而，在与PQ孵育24小时期间释放到HUVEC培养基中的乳酸脱氢酶（LDH）并未增加。许多研究者表明，血清ACE活性的变化反映了血管内皮细胞的损伤。我们在本报告中表明，在PQ诱导的内皮损伤早期，当LDH释放尚未增加时，ACE就已经降低。