Department of Nutritional Sciences, Faculty of Health and Welfare Science, Nayoro City University, Kita 8-1, Nishi 4, Nayoro, Hokkaido, Japan.
Br J Nutr. 2012 Feb;107(4):485-92. doi: 10.1017/S0007114511003229. Epub 2011 Jul 15.
We investigated whether the feeding of high H2-generating dietary fibre and resistant starch (RS) could suppress hepatic ischaemia-reperfusion (IR) injury, which results from oxidative stress, in rats fed a pectin (Pec) or high-amylose maize starch (HAS) diet. Male Sprague-Dawley rats were fed a control (C) diet, with or without Pec (0-5 % Pec) or HAS (0-30 % HAS) supplementation for 7 d. Portal H2 concentration showed a significant dose-dependent increase with the amount of Pec or HAS supplementation. Plasma alanine and aspartate aminotransferase activities remarkably increased in the C rats (5 % cellulose) due to IR treatment, while it decreased significantly or showed tendencies to decrease in 5 % Pec and 20 % HAS diet-fed rats. The hepatic oxidised glutathione (GSSG):total glutathione ratio increased significantly in IR rats maintained on the C diet compared with sham-operated rats. On the other hand, reduced glutathione (GSH):total glutathione and GSH:GSSG ratios decreased significantly. The GSSG:total glutathione ratio that increased due to IR treatment decreased significantly on HAS and Pec intake, while GSH:total glutathione and GSH:GSSG ratios increased significantly. Hepatic sinusoids of IR rats fed the C diet were occluded, but those of IR rats fed the Pec diet were similar to those in the sham-operated rats. In conclusion, we found that Pec or HAS, which enhance H2 generation in the large intestine, alleviated hepatic IR injury. The present study demonstrates another physiological significance of dietary fibre and RS.
我们研究了高产氢膳食纤维和抗性淀粉（RS）的喂养是否可以抑制大鼠的肝缺血再灌注（IR）损伤，这种损伤是由氧化应激引起的，这些大鼠分别喂食果胶（Pec）或高直链玉米淀粉（HAS）饮食。雄性 Sprague-Dawley 大鼠分别喂食对照（C）饮食，或添加 0-5%Pec 或 0-30%HAS 的饮食，持续 7 天。门静脉 H2 浓度随 Pec 或 HAS 补充量呈显著的剂量依赖性增加。由于 IR 处理，C 组（5%纤维素）大鼠的血浆丙氨酸和天冬氨酸转氨酶活性显著升高，而 5%Pec 和 20%HAS 饮食喂养的大鼠的这些酶活性显著降低或有降低的趋势。与假手术大鼠相比，C 饮食喂养的 IR 大鼠的肝氧化型谷胱甘肽（GSSG）：总谷胱甘肽比值显著增加。另一方面，还原型谷胱甘肽（GSH）：总谷胱甘肽和 GSH：GSSG 比值显著降低。由于 IR 处理而增加的 GSSG：总谷胱甘肽比值在摄入 HAS 和 Pec 后显著降低，而 GSH：总谷胱甘肽和 GSH：GSSG 比值显著增加。C 饮食喂养的 IR 大鼠的肝窦被阻塞，但 Pec 饮食喂养的 IR 大鼠的肝窦与假手术大鼠相似。总之，我们发现，在大肠中产生大量氢气的 Pec 或 HAS 可以减轻肝 IR 损伤。本研究证明了膳食纤维和 RS 的另一个生理意义。
