Min Xiang, Tang Minjian, Jiao Yanpeng, Zhou Changren
a Department of Materials Science and Engineering , Jinan University , Guangzhou , 510632 , P. R. China.
J Biomater Sci Polym Ed. 2012;23(11):1421-35. doi: 10.1163/092050611X582858. Epub 2012 May 8.
In this study, chitosan/poly(ϵ-caprolactone) (PCL) blend films with different mass ratios were prepared by spin coating with chitosan/PCL mixture solution. The surface topography and hydrophilicity of the blend films were examined with atomic force microscopy and drop shape analysis, respectively. The adsorption of fibronectin (FN) on the blend films surface was detected by quartz crystal microbalance (QCM). The adhesion and cell morphology of mouse fibroblast cells (3T3) on the films were also evaluated. Metabolic activity of 3T3 cells cultured on the films was assessed by the MTT assay. The results showed that the topography and hydrophilicity of the blend films were largely related to the mass ratio of chitosan and PCL. The amount of adsorbed FN and 3T3 on the blend films decreased with the increase in chitosan content. In contrast, adhesion and cytoskeleton reorganization of 3T3 cells on the films with lower ratio of chitosan appeared to be largely inhibited.
在本研究中，通过用壳聚糖/聚己内酯（PCL）混合溶液旋涂制备了不同质量比的壳聚糖/聚己内酯（PCL）共混膜。分别用原子力显微镜和液滴形状分析法研究了共混膜的表面形貌和亲水性。用石英晶体微天平（QCM）检测纤连蛋白（FN）在共混膜表面的吸附情况。还评估了小鼠成纤维细胞（3T3）在膜上的粘附和细胞形态。通过MTT法评估在膜上培养的3T3细胞的代谢活性。结果表明，共混膜的形貌和亲水性在很大程度上与壳聚糖和PCL的质量比有关。随着壳聚糖含量的增加，共混膜上吸附的FN和3T3的量减少。相反，壳聚糖比例较低的膜上3T3细胞的粘附和细胞骨架重组似乎受到很大抑制。
