Rouxhet L, Duhoux F, Borecky O, Legras R, Schneider Y J
Laboratoire de Physique et de Chimie des Hauts Polymères, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
J Biomater Sci Polym Ed. 1998;9(12):1279-304. doi: 10.1163/156856298x00398.
The effect of alkaline hydrolysis on several surface properties of poly(hydroxybutyrate-hydroxyvalerate) (92/8) (PHB/HV) and poly(epsilon-caprolactone) (PCL) films and of poly(ethylene terephtalate) (PET) track-etched membranes have been characterized, as well as the adsorption of three proteins normally encountered by mammalian cells in vivo, namely albumin, collagen, and fibronectin. The water contact angle decreases and the number of -COOH functions accessible to a chemical reaction at the surface of PCL increases with alkaline hydrolysis. Analysis by atomic force microscopy pictures reveals a change in surface morphology. The modifications of surface properties are correlated with a two times increase of the adsorption of three radiolabelled proteins. The hydrolysis results in a slight increase in the water contact angle of one face of the PHB/HV film and a sharp increase in the number of -COOH functions. Important morphology changes are also induced. The adsorption of the radiolabelled proteins is almost 100 times higher on the hydrolyzed polymer than on the native surface. The increase in hydrophilicity of different PET batches correlates to an increase in the number of -COOH functions. Nevertheless, the surface chemical composition and rugosity are constant and no significant difference in the amount of radiolabelled fibronectin adsorbed on the different surfaces is detectable. In conclusion, the effect of hydrolysis on the surface properties of each of the polyesters studied as well as the proteins adsorption on the different surfaces are different. The results strongly support the hypothesis that, in the system studied, parameters other than hydrophilicity influence protein adsorption: the main parameters that might play a role are the total surface area accessible to the proteins, as well as the surface chemical composition.
已对碱性水解对聚（3-羟基丁酸酯-3-羟基戊酸酯）（92/8）（PHB/HV）和聚己内酯（PCL）薄膜以及聚对苯二甲酸乙二酯（PET）径迹蚀刻膜的几种表面性质的影响进行了表征，同时也研究了哺乳动物细胞在体内通常会遇到的三种蛋白质，即白蛋白、胶原蛋白和纤连蛋白的吸附情况。随着碱性水解，PCL表面的水接触角减小，可用于化学反应的-COOH官能团数量增加。通过原子力显微镜图像分析揭示了表面形态的变化。表面性质的改变与三种放射性标记蛋白质吸附量增加两倍相关。水解导致PHB/HV薄膜一面的水接触角略有增加，-COOH官能团数量急剧增加。同时也诱导了重要的形态变化。水解后的聚合物表面上放射性标记蛋白质的吸附量几乎比天然表面高100倍。不同批次PET亲水性的增加与-COOH官能团数量的增加相关。然而，表面化学成分和粗糙度保持不变，不同表面上吸附的放射性标记纤连蛋白量没有可检测到的显著差异。总之，水解对所研究的每种聚酯表面性质以及不同表面上蛋白质吸附的影响是不同的。结果有力地支持了这样的假设：在所研究的体系中，除亲水性外的其他参数会影响蛋白质吸附，可能起作用的主要参数是蛋白质可及的总表面积以及表面化学成分。
