Department of Poultry Science, Auburn University, Auburn, AL 36849-5416, USA.
J Virol Methods. 2011 Oct;177(1):75-9. doi: 10.1016/j.jviromet.2011.06.022. Epub 2011 Jul 5.
Avian reoviruses (ARVs) are an important cause of economic losses in commercial poultry. A TaqMan real-time RT-PCR assay for detecting of ARVs was developed. The primer-probe set was from the conserved region of ARV S4 genome segment. Real-time RT-PCR detected ARV strains including CO8 and ss412 strains, which belonged to different serological subgroups, and the test had no cross-reaction with other avian viruses. The detection limit of this assay was 5 ARV genome copies per 5 μl and was 150 times more sensitive than traditional RT-PCR. Statistical analyses indicated excellent reproducibility. For ARV strain 2408, a titer of 50% embryo infection dose and 50% tissue culture infectious dose equivalent to 3.9 ± 0.8, and 2.9 ± 0.3 ARV genome copies, respectively. This test was rapid, specific, and sensitive for the detection of ARVs and will be useful in veterinary diagnostic laboratories and for the quantitation of vaccine viruses for pharmaceutical companies.
禽呼肠孤病毒（ARV）是商业家禽业经济损失的重要原因。开发了一种 TaqMan 实时 RT-PCR 检测 ARV 的方法。该引物-探针集来自 ARV S4 基因组片段的保守区域。实时 RT-PCR 检测到包括 CO8 和 ss412 株在内的 ARV 株，它们属于不同的血清学亚群，该检测与其他禽病毒无交叉反应。该检测方法的检测限为每 5 μl 5 ARV 基因组拷贝，比传统 RT-PCR 灵敏 150 倍。统计分析表明该方法具有极好的重现性。对于 ARV 株 2408，50%胚体感染剂量和 50%组织培养感染剂量的效价分别相当于 3.9 ± 0.8 和 2.9 ± 0.3 ARV 基因组拷贝。该检测方法快速、特异、灵敏，可用于兽医诊断实验室和制药公司疫苗病毒的定量。
