Laboratory of Fish Reproduction, Aquaculture Center, São Paulo State University, Jaboticabal, SP, Brazil.
Micron. 2011 Dec;42(8):892-7. doi: 10.1016/j.micron.2011.06.008. Epub 2011 Jun 24.
The present study examined the testicular structure and the seminal pathway in freshwater fish Leporinus macrocephalus (Garavello and Britski, 1988). Twenty-five specimens of this species were studied. Testicular structure was analyzed using light and transmission electron microscopy. The testicular main ducts were examined by means of conventional histology, corrosion-cast technique and scanning electron microscopy. Additional techniques were applied for polysaccharides histochemistry and immunohistochemistry for androgen receptor. The testicular parenchyma was classified as the anastomosing tubular testis type with spermatogonia occurring along the lengths of the seminiferous tubules. The seminiferous tubules emptied directly into the testicular main ducts. The wall of the testicular main ducts in L. macrocephalus consisted of three layers: epithelium, connective tissue and peritoneum. The epithelium changed from simple cuboidal to pseudostratified. The histochemical analysis revealed the presence of granules PAS positive in the epithelial cells. The immunoreactivity to androgen receptor was noted in the testicular main ducts through all cytoplasmic areas of epithelial cells.
本研究检查了淡水鱼 Leporinus macrocephalus（Garavello 和 Britski，1988）的睾丸结构和精液途径。研究了 25 个该物种的标本。使用光镜和透射电子显微镜分析了睾丸结构。通过常规组织学、腐蚀铸造技术和扫描电子显微镜检查了睾丸主导管。还应用了多糖组织化学和雄激素受体免疫组织化学等技术。睾丸实质被分类为具有生精细胞的吻合管状睾丸类型，生精细胞沿着曲细精管的长度发生。曲细精管直接排入睾丸主导管。L. macrocephalus 的睾丸主导管壁由三层组成：上皮、结缔组织和腹膜。上皮从简单的立方上皮变为假复层上皮。组织化学分析显示上皮细胞中存在 PAS 阳性颗粒。雄激素受体的免疫反应性在睾丸主导管的所有上皮细胞质区域都有被观察到。
