Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles, State University of Ceará, Fortaleza, CE, Brazil.
Mol Cell Endocrinol. 2011 Oct 15;345(1-2):38-47. doi: 10.1016/j.mce.2011.07.006. Epub 2011 Jul 6.
This study evaluated the levels of c-kit mRNA in goat follicles and the effects of kit ligand (KL) on the in vitro development of cultured preantral follicles. Preantral follicles isolated from goat ovarian cortex were cultured for 18 days in α-MEM(+) supplemented with KL (0, 50 or 100 ng/mL) in the absence or presence of follicle stimulating hormone (FSH). Real-time PCR showed that c-kit mRNA was higher in primordial and primary follicles than in secondary stage. Regarding the culture, KL addition in the absence of FSH improved the follicular survival, antrum formation, oocyte growth and meiotic resumption. KL-positive effects were not observed in the presence of FSH. In conclusion, c-kit mRNAs are detected in all follicular categories. KL promotes the survival and antral cavity formation of caprine preantral follicles after in vitro culture, as well as the growth and meiotic resumption of their oocytes in the absence of FSH.
本研究评估了 c-kit mRNA 在山羊卵泡中的水平，以及 kit 配体 (KL) 对体外培养的山羊腔前卵泡发育的影响。从山羊卵巢皮质中分离出腔前卵泡，在α-MEM(+)中培养 18 天，KL(0、50 或 100ng/mL)存在或不存在卵泡刺激素 (FSH) 的情况下。实时 PCR 显示，c-kit mRNA 在原始和初级卵泡中的表达高于次级阶段。关于培养，在没有 FSH 的情况下添加 KL 可提高卵泡存活率、腔形成、卵母细胞生长和减数分裂恢复。在存在 FSH 的情况下未观察到 KL 的阳性作用。总之，在所有卵泡类别中均检测到 c-kit mRNAs。KL 在体外培养后促进山羊腔前卵泡的存活和腔形成，以及其卵母细胞的生长和减数分裂恢复，而无需 FSH。
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