Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland.
Neuroimage. 2011 Sep 15;58(2):572-8. doi: 10.1016/j.neuroimage.2011.06.074. Epub 2011 Jul 5.
Changes in intracellular Na(+) concentration underlie essential neurobiological processes, but few reliable tools exist for their measurement. Here we characterize a new synthetic Na(+)-sensitive fluorescent dye, Asante Natrium Green (ANG), with unique properties. This indicator was excitable in the visible spectrum and by two-photon illumination, suffered little photobleaching and located to the cytosol were it remained for long durations without noticeable unwanted effects on basic cell properties. When used in brain tissue, ANG yielded a bright fluorescent signal during physiological Na(+) responses both in neurons and astrocytes. Synchronous electrophysiological and fluorometric recordings showed that ANG produced accurate Na(+) measurement in situ. This new Na(+) indicator opens innovative ways of probing neuronal circuits.
细胞内钠离子浓度的变化是神经生物学过程的基础，但目前几乎没有可靠的工具来测量它。在这里，我们描述了一种新型的合成钠离子敏感荧光染料，Asante Natrium Green（ANG），它具有独特的性质。这种指示剂在可见光谱和双光子激发下具有激发能力，光漂白程度低，定位在细胞质中，可以长时间保持稳定，对基本细胞特性几乎没有明显的不良影响。当用于脑组织时，ANG 在神经元和星形胶质细胞的生理钠离子反应中产生明亮的荧光信号。同步电生理和荧光记录显示，ANG 可以在原位进行准确的钠离子测量。这种新型钠离子指示剂为探测神经元回路开辟了新的途径。
