Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
FEBS Lett. 2011 Aug 4;585(15):2526-32. doi: 10.1016/j.febslet.2011.07.008. Epub 2011 Jul 13.
MazF is an mRNA interferase which cleaves mRNAs at a specific sequence. Here, we show that in contrast to MazF-ec from Escherichia coli, which specifically cleaves ACA sequences, MazF-bs from Bacillus subtilis is an mRNA interferase that specifically cleaves a five-base sequence, UACAU. MazF homologues widely prevailing in Gram-positive bacteria were found to be highly homologous to MazF-bs, suggesting that they may also have similar cleavage specificity. This cleavage site is over-represented in the B. subtilis genes associated with biosynthesis of secondary metabolites, suggesting that MazF-bs may be involved in the regulation of the production of secondary metabolites.
MazF 是一种 mRNA 内切酶，可在特定序列处切割 mRNAs。在这里，我们表明，与特异性切割 ACA 序列的大肠杆菌 MazF-ec 不同，枯草芽孢杆菌 MazF-bs 是一种特异性切割五碱基序列 UACAU 的 mRNA 内切酶。在革兰氏阳性菌中广泛存在的 MazF 同源物与 MazF-bs 高度同源，表明它们可能也具有类似的切割特异性。该切割位点在与次生代谢物生物合成相关的枯草芽孢杆菌基因中过度表达，表明 MazF-bs 可能参与了次生代谢物产生的调控。
