静电纺丝亚微米纤维对磷酸钙骨水泥支架的机械性能和脐带干细胞成骨分化的影响。
Effects of electrospun submicron fibers in calcium phosphate cement scaffold on mechanical properties and osteogenic differentiation of umbilical cord stem cells.
机构信息
Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
出版信息
Acta Biomater. 2011 Nov;7(11):4037-44. doi: 10.1016/j.actbio.2011.06.046. Epub 2011 Jul 1.
Fibrous scaffolds are promising for tissue engineering because of the high surface area and fibrous features mimicking the extracellular matrix in vivo. Calcium phosphate cements (CPCs) can be injected and self-set in the bone defect. A literature search revealed that there have been no reports on stem cell seeding on CPC containing electrospun submicron fibers. The objective of this study was to investigate for the first time the effects of electrospun fibers in CPC on mechanical properties and human umbilical cord mesenchymal stem cell (hUCMSC) proliferation, osteogenic differentiation and mineralization. Poly(D,L-lactide-co-glycolide) fibers were made via an electrospinning technique to yield an average fiber diameter of 650 nm. The fibers were incorporated into CPC consisting of tetracalcium phosphate, dicalcium phosphate anhydrous and chitosan lactate. Fiber volume fractions were 0%, 2.5%, 5% and 10%. CPC with 10% fibers had a flexural strength that was twice that of CPC without fibers, and a work-of-fracture (toughness) that was an order of magnitude larger than that of CPC without fibers. hUCMSCs proliferated rapidly and synthesized bone minerals when attached to the electrospun fiber-CPC scaffolds. Alkaline phosphatase, osteocalcin and collagen I expressions of hUCMSCs were doubled, while mineralization was increased by 40%, when fiber volume fraction in CPC was increased from 0% to 10%. The enhanced cell function was attributed to the high surface area and biomimetic features of the fiber-CPC scaffold. In conclusion, incorporating submicron fibers into CPC greatly improved the strength and toughness of the CPC. Creating submicron fibrous features in CPC was a useful method for enhancing the osteogenic differentiation and mineralization of stem cells. The novel electrospun fiber-CPC-hUCMSC construct is promising for stem cell delivery and bone tissue engineering.
纤维支架在组织工程中很有前景，因为它们具有高的比表面积和纤维状特征，模仿了体内的细胞外基质。磷酸钙水泥（CPCs）可以在骨缺损处注射并自凝固。文献检索表明，目前还没有关于在含有静电纺丝亚微米纤维的 CPC 上接种干细胞的报道。本研究的目的是首次研究静电纺丝纤维对 CPC 的机械性能以及人脐带间充质干细胞（hUCMSC）增殖、成骨分化和矿化的影响。通过静电纺丝技术制备聚（D，L-丙交酯-共-乙交酯）纤维，平均纤维直径为 650nm。将纤维掺入由磷酸四钙、磷酸二氢钙无水物和乳酸壳聚糖组成的 CPC 中。纤维体积分数分别为 0%、2.5%、5%和 10%。含有 10%纤维的 CPC 的弯曲强度是不含纤维的 CPC 的两倍，断裂功（韧性）是不含纤维的 CPC 的一个数量级。当 hUCMSCs 附着在静电纺丝纤维-CPC 支架上时，它们会快速增殖并合成骨矿物质。当 CPC 中的纤维体积分数从 0%增加到 10%时，hUCMSCs 的碱性磷酸酶、骨钙素和 I 型胶原的表达增加了一倍，而矿化增加了 40%。细胞功能的增强归因于纤维-CPC 支架的高比表面积和仿生特征。总之，将亚微米纤维掺入 CPC 中大大提高了 CPC 的强度和韧性。在 CPC 中制造亚微米纤维特征是增强干细胞成骨分化和矿化的有效方法。新型静电纺丝纤维-CPC-hUCMSC 构建体有望用于干细胞递送和骨组织工程。
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