The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Laboratory of Intestinal Adaptation and Recovery, Haifa 35254, Israel.
J Pediatr Surg. 2011 Jul;46(7):1353-60. doi: 10.1016/j.jpedsurg.2010.09.051.
BACKGROUND/PURPOSE: Fatty acids from fish oil (omega-3 polyunsaturated fatty acids, 3PUFAs) are emerging as powerful yet safe disease-modifying nutrients and are protective in severe critical care conditions including ischemia-reperfusion (IR) injury. The purpose of the present study was to examine the effects of 3PUFAs on intestinal structural changes, enterocyte proliferation, and apoptosis after intestinal IR in a rat.
Male rats were divided into three experimental groups: sham rats underwent laparotomy, IR rats underwent occlusion of both superior mesenteric artery and portal vein for 30 minutes followed by 48 hours of reperfusion, and 3PUFA-treated IR (IR-3PUFA) rats underwent IR and were treated with Omegaven (Fresenius Kabi, Bad Homburg, Germany) given intraperitoneally at a dose of 1 mL twice a day. Intestinal structural changes (Park injury score, overall bowel and mucosal weight, mucosal DNA and protein, villus height and crypt depth, cell proliferation, and apoptosis) were determined 48 hours after IR. Real-time polymerase chain reaction (PCR) was used to determine the level of bax and bcl-2 messenger RNA.
A significant decrease in bowel and mucosal weight was observed in the ileum of untreated IR rats compared with sham animals. Forty-eight hours after IR, cell apoptosis remained increased in the jejunum and ileum, which coincided with increased bax/bcl-2 ratio. Cell proliferation was increased 48 hours after IR, suggesting tissue repair. Treatment with Omegaven resulted in a significant increase in bowel and mucosal weight in the jejunum and ileum, villus height in the jejunum and ileum, and crypt depth in the jejunum compared with untreated IR animals. IR-3PUFA rats also demonstrated a significantly lower Park injury score in the jejunum and ileum as well as a lower apoptotic index in the ileum compared with untreated IR animals.
Parenteral Omegaven administration decreases the intestinal mucosal injury and inhibits enterocyte apoptosis after intestinal IR in a rat.
背景/目的：鱼油中的脂肪酸（ω-3 多不饱和脂肪酸，3PUFAs）作为强大而安全的疾病修饰营养素正在出现，并在包括缺血再灌注（IR）损伤在内的严重重症监护条件下具有保护作用。本研究的目的是在大鼠中研究 3PUFAs 对肠道结构变化、肠上皮细胞增殖和 IR 后细胞凋亡的影响。
雄性大鼠分为三组实验：假手术组大鼠接受剖腹手术，IR 组大鼠夹闭肠系膜上动脉和门静脉 30 分钟，然后再灌注 48 小时，3PUFA 处理的 IR（IR-3PUFA）组大鼠接受 IR，并每天两次腹腔内给予 Omegaven（Fresenius Kabi，德国巴特洪堡）1 毫升。IR 后 48 小时，通过 Park 损伤评分、全肠和黏膜重量、黏膜 DNA 和蛋白质、绒毛高度和隐窝深度、细胞增殖和细胞凋亡来确定肠道结构变化。实时聚合酶链反应（PCR）用于确定 bax 和 bcl-2 信使 RNA 的水平。
与假手术动物相比，未经处理的 IR 大鼠的回肠肠壁和黏膜重量明显减轻。IR 后 48 小时，空肠和回肠的细胞凋亡仍然增加，同时 bax/bcl-2 比值增加。IR 后 48 小时，细胞增殖增加，提示组织修复。与未经处理的 IR 动物相比，Omegaven 治疗可使空肠和回肠的肠壁和黏膜重量、空肠和回肠的绒毛高度以及空肠的隐窝深度显著增加。IR-3PUFA 大鼠的空肠和回肠的 Park 损伤评分以及回肠的细胞凋亡指数也明显低于未经处理的 IR 大鼠。
肠外给予 Omegaven 可减轻大鼠肠黏膜损伤并抑制肠上皮细胞凋亡。
