Gutiérrez-Sánchez Gaizka, García-Alonso Ignacio, Gutiérrez Sáenz de Santa María Jorge, Alonso-Varona Ana, Herrero de la Parte Borja
Department of Surgery and Radiology and Physical Medicine, University of The Basque Country, ES48940 Leioa, Biscay, Spain.
Interventional Radiology Research Group, Biocruces Bizkaia Health Research Institute, ES48903 Barakaldo, Biscay, Spain.
Antioxidants (Basel). 2021 May 27;10(6):853. doi: 10.3390/antiox10060853.
Intestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with a high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most of the damage is triggered by the restoration of flow and the arrival of cytokines and reactive oxygen species (ROS), among others. Inactivation of these molecules before tissue reperfusion could reduce intestinal damage. The aim of this work was to analyze the preventive effect of allopurinol and nitroindazole on intestinal mucosal damage after i-IRI. Wag/RijHsd rats were subjected to i-IRI by clamping the superior mesenteric artery (for 1 or 2 h) followed by a 30 min period of reperfusion. Histopathological intestinal damage (HID) was assessed by microscopic examination of histological sections obtained from injured intestine. HID was increased by almost 20% by doubling the ischemia time (from 1 to 2 h). Nitroindazole reduced HID in both the 1 and 2 h period of ischemia by approximately 30% and 60%, respectively ( < 0.001). Our preliminary results demonstrate that nitroindazole has a preventive/protective effect against tissue damage in the early stages of i-IRI. However, to better understand the molecular mechanisms underlying this phenomenon, further studies are needed.
肠缺血再灌注损伤（i-IRI）是一种死亡率很高的罕见疾病，由肠段血流丧失引起。大部分损伤是由血流恢复以及细胞因子和活性氧（ROS）等物质的到来引发的。在组织再灌注前使这些分子失活可减轻肠道损伤。这项工作的目的是分析别嘌呤醇和硝唑对i-IRI后肠黏膜损伤的预防作用。通过夹闭肠系膜上动脉（持续1或2小时），随后进行30分钟的再灌注，使Wag/RijHsd大鼠遭受i-IRI。通过对取自受损肠道的组织切片进行显微镜检查来评估组织病理学肠道损伤（HID）。将缺血时间加倍（从1小时增加到2小时）使HID增加了近20%。硝唑在缺血1小时和2小时期间分别使HID降低了约30%和60%（<0.001）。我们的初步结果表明，硝唑对i-IRI早期的组织损伤具有预防/保护作用。然而，为了更好地理解这一现象背后的分子机制，还需要进一步研究。
