Department of Emergency Medicine, Division of Pediatric Emergency Medicine, Loma Linda University Children's Hospital, Loma Linda, California, USA.
Pediatr Neurol. 2011 Aug;45(2):77-82. doi: 10.1016/j.pediatrneurol.2011.04.004.
We investigated whether morphine plays a neuroprotective role in a neonatal rat pup model of bilateral carotid artery occlusion with hypoxia. At postnatal day 10, rats received either morphine (n = 7), naloxone (n = 7), or saline placebo (n = 15) after hypoxic-ischemic injury. Survival (days), weight gain and animal testing (negative geotaxis, surface righting, and rotarod) were compared between treatment groups. Lesion volume was delineated with magnetic resonance imaging at days 7 and 28-57 after injury. Survival in rats treated with morphine, naloxone, or saline was, respectively, 14, 29, and 73%. Median number of days of survival after bilateral carotid artery occlusion with hypoxia treated with morphine was 4 (95% confidence interval 4 to 22), with naloxone was 3 (95% confidence interval -1.4 to 21), and with placebo was 28 (95% confidence interval 18 to 28). There were no statistically significant differences in magnetic resonance imaging-derived ischemic lesion volumes, weight gain, or behavioral testing measures between the groups. Morphine was ineffective as a neuroprotectant in rat pups with severe hypoxic-ischemic injury and may have contributed to their decreased survival.
我们研究了吗啡在伴有缺氧的双侧颈总动脉闭塞新生大鼠模型中是否具有神经保护作用。在生后第 10 天，缺氧缺血损伤后，大鼠分别接受吗啡（n = 7）、纳洛酮（n = 7）或生理盐水安慰剂（n = 15）治疗。在治疗组之间比较了存活率（天）、体重增加和动物测试（负趋地性、表面翻正和转棒）。损伤后第 7 天和第 28-57 天用磁共振成像描绘损伤体积。用吗啡、纳洛酮或生理盐水治疗的大鼠的存活率分别为 14%、29%和 73%。用吗啡治疗的双侧颈总动脉闭塞伴缺氧大鼠的中位存活天数为 4 天（95%置信区间为 4 至 22 天），用纳洛酮治疗的为 3 天（95%置信区间为 -1.4 至 21 天），用安慰剂治疗的为 28 天（95%置信区间为 18 至 28 天）。磁共振成像衍生的缺血性损伤体积、体重增加或行为测试指标在各组之间无统计学显著差异。吗啡在严重缺氧缺血损伤的大鼠幼仔中作为神经保护剂无效，并且可能导致其存活率降低。
