Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Barcelona, Spain.
Biosens Bioelectron. 2011 Sep 15;27(1):113-8. doi: 10.1016/j.bios.2011.06.026. Epub 2011 Jun 28.
This work reports on the fabrication and performance of a simple amperometric immunosensor device to be potentially used for the detection of serum anti-citrullinated peptide antibodies (ACPAs), which are specific for rheumatoid arthritis (RA) autoimmune disease. Sera of RA patients contain antibodies to different citrullinated peptides and proteins such as fibrin or filaggrin. Herein, a chimeric fibrin-filaggrin synthetic peptide (CFFCP1) was used as a recognition element anchored to the surface of a multiwalled carbon nanotube-polystyrene (MWCNT-PS) based electrochemical transducer. The transducer fabrication process is described in detail together with its successful electrochemical performance in terms of repeatability and reproducibility of the corresponding amperometric response. The resulting immunosensor approach was initially tested in sera of rabbits previously inoculated with the synthetic peptide and eventually applied to the detection of ACPAs in human sera. A comparative study was carried out using control serum from a blood donor, which demonstrated the selectivity of the immunosensor response and its sensitivity for the detection of anti-CFFCP1 antibodies present in RA patients.
这项工作报道了一种简单的电流免疫传感器设备的制造和性能，该设备可用于检测血清抗瓜氨酸化肽抗体（ACPAs），这些抗体是类风湿关节炎（RA）自身免疫疾病的特异性抗体。RA 患者的血清中含有针对不同瓜氨酸化肽和蛋白质的抗体，如纤维蛋白或角蛋白。在此，使用纤维蛋白-角蛋白嵌合合成肽（CFFCP1）作为识别元件，锚定在基于多壁碳纳米管-聚苯乙烯（MWCNT-PS）的电化学传感器的表面上。详细描述了传感器的制造过程，并展示了其在相应电流响应的重复性和重现性方面的电化学性能。该免疫传感器方法最初在预先用合成肽接种的兔子血清中进行了测试，最终应用于检测人类血清中的 ACPAs。使用来自献血者的对照血清进行了比较研究，证明了免疫传感器响应的选择性及其对 RA 患者中存在的抗 CFFCP1 抗体的检测灵敏度。
