University of Wisconsin-Madison Biotechnology Center and Department of Biochemistry, 425 Henry Mall, Madison, WI 53706, USA.
Curr Opin Plant Biol. 2011 Oct;14(5):507-11. doi: 10.1016/j.pbi.2011.06.008. Epub 2011 Jul 18.
Protein phosphorylation is a major post-translational modification in plants crucial for the regulation of diverse cellular functions. In the early stages of this field, efforts were focused on the qualitative detection, identification, and cataloging of in vivo protein phosphorylation sites. Recently these studies have advanced into utilizing quantitative mass spectrometric measurements, capable of dynamically monitoring changes in phosphorylation levels in response to genetic and environmental alterations. This review will highlight current untargeted and targeted mass spectral technologies used for quantitative phosphoproteome measurements in plants, and provide a discussion of these phosphorylation changes in relation to important biological events.
蛋白质磷酸化是植物中一种重要的翻译后修饰,对调节多种细胞功能至关重要。在该领域的早期阶段,人们主要致力于定性检测、鉴定和编目体内蛋白质磷酸化位点。最近,这些研究已经发展到利用定量质谱测量技术,能够动态监测磷酸化水平的变化,以响应遗传和环境的改变。本文综述了目前用于植物定量磷酸蛋白质组学测量的非靶向和靶向质谱技术,并讨论了这些磷酸化变化与重要生物学事件的关系。