Zhang Lan-lan, Cao Qiong, Hu Zi-you, Yan Xiao-hui, Wu Bing-yi
Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(7):1200-3.
To study the effect of quercetin on the proliferation of neural stem cells in the subventricular zone (SVZ) of rats after focal cerebral ischemia.
An adult rat model of middle cerebral artery occlusion (MCAO) model was established by placement of an intraluminal filament at the origin of the MCA. Quercetin was administered intraperitoneally in the rats at a dose of 50 mg/kg every 3 days starting at 6 h after MCAO, and BrdU (50 mg/kg daily) was also injected intraperitoneally starting at 4 h after MCAO. BrdU-positive cells in the SVZ were counted at 7, 14 and 21 days after MCAO.
Compared with the sham-operated group, the rats in the ischemic model group showed significantly increased BrdU-positive cells in the ipsilateral SVZ 7 days after MCAO, reaching the peak level on day 14 and beginning to decrease on day 21 (P<0.05). The number of ipsilateral BrdU-positive cells in quercetin group was significantly greater than that in the model group on days 7, 14 and 21 (P<0.05), and maintained the high level on day 21.
Quercetin can maintain a high level of neural stem cell proliferation in the SVZ after focal cerebral ischemia in adult rats.
研究槲皮素对成年大鼠局灶性脑缺血后室下区（SVZ）神经干细胞增殖的影响。
通过在大脑中动脉（MCA）起始处插入腔内细丝建立成年大鼠大脑中动脉闭塞（MCAO）模型。MCAO后6小时开始，以50mg/kg的剂量每3天对大鼠进行一次槲皮素腹腔注射，MCAO后4小时开始也腹腔注射BrdU（每日50mg/kg）。在MCAO后7、14和21天对SVZ中的BrdU阳性细胞进行计数。
与假手术组相比，缺血模型组大鼠在MCAO后7天同侧SVZ中的BrdU阳性细胞显著增加，在第14天达到峰值水平，并在第21天开始下降（P<0.05）。在第7、14和21天，槲皮素组同侧BrdU阳性细胞数量显著多于模型组（P<0.05），并在第21天维持在高水平。
槲皮素可使成年大鼠局灶性脑缺血后SVZ中的神经干细胞增殖维持在高水平。
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