Feng Gai-feng, Hu Hai-tao, Jin Hui, Wang Wei-xi, Qian Yi-hua
Department of Human Anatomy and Histoembryology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(7):1236-9.
To study the effects of immunization with the fusion protein CAC (a product of prokaryotic expression of recombinant HBcAg and β-amyloid peptide fusion gene) against the toxicity induced by intrahippocampal injection of aggregated β-amyloid peptide (Aβ) in rats.
SD rats were immunized intraperitoneally with the fusion protein CAC, and the titer of anti-Aβ antibody was evaluated by ELISA. When the titers of the anti-Aβ antibody reached 1:3 000, aggregated Aβ was injected into the CA1 region of the rat hippocampus. Two weeks after Aβ injection, the rats underwent morris water maze test before sacrificed to prepare the brain slices with Congo red and haematoxylin staining.
The titer of anti-Aβ antibody reached 1:3 000 after 5 immunizations with the fusion protein. After Aβ injection, the saline-immunized rats showed a reduced cognitive behavior in the Morris water maze test compared to the CAC-immunized rats. In the saline-immunized rats, the neurons around the site of Aβ injection exhibited obvious cell damages with Aβ deposits and glial infiltration, whereas in CAC-immunized rats, Aβ deposits were significantly reduced or even absent.
Immunization with the fusion protein CAC can inhibit the toxicity induced by intrahippocampal aggregated Aβ injection.
研究融合蛋白CAC（重组乙肝核心抗原与β-淀粉样肽融合基因的原核表达产物）免疫对大鼠海马内注射聚集β-淀粉样肽（Aβ）所致毒性的影响。
用融合蛋白CAC对SD大鼠进行腹腔免疫，采用酶联免疫吸附测定法（ELISA）评估抗Aβ抗体效价。当抗Aβ抗体效价达到1:3000时，将聚集的Aβ注射到大鼠海马CA1区。Aβ注射两周后，对大鼠进行莫里斯水迷宫试验，然后处死大鼠，制备刚果红和苏木精染色的脑切片。
用融合蛋白免疫5次后，抗Aβ抗体效价达到1:3000。Aβ注射后，与用CAC免疫的大鼠相比，用生理盐水免疫的大鼠在莫里斯水迷宫试验中表现出认知行为下降。在用生理盐水免疫的大鼠中，Aβ注射部位周围的神经元出现明显的细胞损伤，伴有Aβ沉积和胶质细胞浸润，而在用CAC免疫的大鼠中，Aβ沉积明显减少甚至消失。
用融合蛋白CAC免疫可抑制海马内注射聚集Aβ所致的毒性。
