Nagayama Y, Russo D, Chazenbalk G D, Wadsworth H L, Rapoport B
Thyroid Molecular Biology Unit, V.A. Medical Center, San Francisco, California 94121.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):1150-6. doi: 10.1016/s0006-291x(05)80906-4.
We constructed a series of TSH-LH/CG receptor chimeras by homologous substitution of relatively small regions of the TSH receptor extracellular domain for the corresponding region of the extracellular domain of the LH/CG receptor. Constructs were stably expressed in Chinese hamster ovary cells. Of the five chimeric receptors, only TSH-LHR-14, which contains mid-region domain C (amino acid residues 171-260) of the extracellular component of the TSH receptor, exhibited TSH binding of relatively high affinity. Consistent with this TSH binding, chimera TSH-LHR-14 was the only one that demonstrated a functional response to TSH stimulation in terms of intracellular cAMP generation. These data indicate that domain C plays a vital role in TSH receptor function.
我们通过将促甲状腺激素（TSH）受体细胞外结构域的相对小区域与促黄体生成素/绒毛膜促性腺激素（LH/CG）受体细胞外结构域的相应区域进行同源替换，构建了一系列TSH-LH/CG受体嵌合体。构建体在中国仓鼠卵巢细胞中稳定表达。在五个嵌合受体中，只有包含TSH受体细胞外成分中间区域结构域C（氨基酸残基171 - 260）的TSH-LHR-14表现出相对高亲和力的TSH结合。与这种TSH结合一致，嵌合体TSH-LHR-14是唯一一个在细胞内cAMP生成方面对TSH刺激表现出功能性反应的。这些数据表明结构域C在TSH受体功能中起至关重要的作用。
