Department of Endocrinology and Metabolism, University of Pisa, Pisa, Italy.
Islets. 2011 Sep-Oct;3(5):231-3. doi: 10.4161/isl.3.5.15940. Epub 2011 Sep 1.
Gestational diabetes (GD) results from insufficient endogenous insulin supply. No information is available on features of islet cells in human GD. Herein, we describe several properties of islets from a woman with GD. Immunohistochemical stainings and EM analyses were performed on pancreatic samples. Islet isolation was achieved by enzymatic dissociation and density gradient centrifugation. Ex vivo insulin secretion was studied in response to fuel secretagogues. Control islets were obtained from matched non-pregnant, non-diabetic women. Total insulin positive area was lower in GD, mainly due to the presence of smaller islets. β-cell apoptosis and the presence of Ki67 positive islet cells were similar in GD and controls, whereas the amount of insulin positive cells in or close to the ducts was decreased in GD. Ex vivo insulin secretion did not differ between GD and non-pregnant, non-diabetic islets. These findings suggest that in this case of human GD there might mainly be a defect of β-cell amount, not due to increased apoptosis, but possibly to insufficient regeneration.
妊娠期糖尿病（GD）是由于内源性胰岛素供应不足引起的。目前尚无关于人类 GD 胰岛细胞特征的信息。在此，我们描述了一位 GD 女性的胰岛的几个特征。对胰腺样本进行免疫组织化学染色和 EM 分析。通过酶解和密度梯度离心进行胰岛分离。研究了对燃料分泌激动剂的体外胰岛素分泌。对照胰岛取自匹配的非妊娠、非糖尿病女性。GD 中的总胰岛素阳性面积较低，主要是由于较小的胰岛存在。GD 和对照组的β细胞凋亡和 Ki67 阳性胰岛细胞的存在相似，而 GD 中靠近导管的胰岛素阳性细胞数量减少。GD 和非妊娠、非糖尿病胰岛的体外胰岛素分泌没有差异。这些发现表明，在这种情况下，人类 GD 可能主要存在β细胞数量的缺陷，不是由于细胞凋亡增加，而是可能由于再生不足。
