对来自溶组织内阿米巴的一个含有酿酒酵母自主复制序列共有序列的重复DNA家族的表征。

Characterisation of a repetitive DNA family from Entamoeba histolytica containing Saccharomyces cerevisiae ARS consensus sequences.

作者信息

Lohia A, Haider N, Biswas B B

机构信息

Department of Biochemistry, Bose Institute, Calcutta India.

出版信息

Gene. 1990 Dec 15;96(2):197-203. doi: 10.1016/0378-1119(90)90253-n.

DOI:10.1016/0378-1119(90)90253-n

PMID:2176638

Abstract

Several repetitive DNA families were identified in Entamoeba histolytica DNA digested with Sau3AI. Characterisation of one of these repetitive DNA families showed the presence of multiple copies of Saccharomyces cerevisiae autonomously replicating sequence (ARS) core consensus sequences. The E. histolytica ARS consensus sequences allowed a yeast-integrating plasmid, YIP5, to replicate autonomously in S. cerevisiae. A 'bent DNA' fragment was located in one member of this E. histolytica repetitive DNA family.

摘要

在用Sau3AI消化的溶组织内阿米巴DNA中鉴定出了几个重复DNA家族。对其中一个重复DNA家族的特征分析表明，存在多个酿酒酵母自主复制序列（ARS）核心共有序列的拷贝。溶组织内阿米巴的ARS共有序列使酵母整合质粒YIP5能够在酿酒酵母中自主复制。一个“弯曲DNA”片段位于这个溶组织内阿米巴重复DNA家族的一个成员中。