Bouton A H, Stirling V B, Smith M M
Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908.
Yeast. 1987 Jun;3(2):107-15. doi: 10.1002/yea.320030207.
We have previously identified an autonomously replicating segment (ARS) near the 3' end of the histone H4 gene at the copy-I H3-H4 locus. We have now searched for additional autonomously replicating segments and sequences homologous with the ARS core consensus sequence near the copy-II histone H4 gene and both of the histone H3 genes. No new ARS elements were identified by functional cloning assays. However, several matches to the ARS core consensus element were found within the DNA sequences of the copy-I and copy-II genes. An exact match to the ARS core consensus was identified in the region downstream from the copy-I histone H3 gene and a set of sequences with weak homology was also located within the copy-II region. However, restriction fragments including these sequences did not demonstrate ARS activity on a plasmid in transformed cells.
我们之前在组蛋白H4基因3'端附近的拷贝-I H3-H4基因座处鉴定出一个自主复制序列（ARS）。我们现在在拷贝-II组蛋白H4基因以及两个组蛋白H3基因附近搜索了其他自主复制序列以及与ARS核心共有序列同源的序列。通过功能克隆分析未鉴定到新的ARS元件。然而，在拷贝-I和拷贝-II基因的DNA序列中发现了几个与ARS核心共有元件匹配的序列。在拷贝-I组蛋白H3基因下游区域鉴定出了与ARS核心共有序列的精确匹配，并且在拷贝-II区域内也定位到了一组具有弱同源性的序列。然而，包含这些序列的限制性片段在转化细胞的质粒上并未表现出ARS活性。
