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从分枝杆菌中提取完整、纯净 RNA 的方法。

A method to extract intact and pure RNA from mycobacteria.

机构信息

Combi Chem Bio Resource Center, Organic Chemical Division, National Chemical Laboratory, Pune, Maharashtra 411008, India.

出版信息

Anal Biochem. 2011 Oct 15;417(2):286-8. doi: 10.1016/j.ab.2011.06.028. Epub 2011 Jun 28.

DOI:10.1016/j.ab.2011.06.028
PMID:21767523
Abstract

We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.

摘要

我们描述了一种高产、简单且无气溶胶的从分枝杆菌中分离 RNA 的方案，该方案无需复杂的仪器。该方法从 10(7)个细胞中提取了 50 μg RNA，比最近报道的方法高 50 倍。我们的方法可以从需氧生长的细菌以及体外缺氧诱导的休眠杆菌和感染巨噬细胞内的分枝杆菌中提取总 RNA。

相似文献

1
A method to extract intact and pure RNA from mycobacteria.从分枝杆菌中提取完整、纯净 RNA 的方法。
Anal Biochem. 2011 Oct 15;417(2):286-8. doi: 10.1016/j.ab.2011.06.028. Epub 2011 Jun 28.
2
Isolation of RNA from mycobacteria grown under in vitro and in vivo conditions.
FEMS Microbiol Lett. 2000 May 15;186(2):177-80. doi: 10.1111/j.1574-6968.2000.tb09100.x.
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A method for the isolation of pure intact RNA from mycobacteria.
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[A new method for the disruption of cell walls of gram-positive bacteria and mycobacteria on the point of nucleic acid extraction: sand method].[一种在核酸提取环节破坏革兰氏阳性菌和分枝杆菌细胞壁的新方法：砂磨法]
Mikrobiyol Bul. 2016 Jan;50(1):34-43. doi: 10.5578/mb.10685.
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Step-wise isolation of RNA and DNA from mycobacteria.从分枝杆菌中逐步分离RNA和DNA。
Int J Lepr Other Mycobact Dis. 1986 Sep;54(3):409-15.
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A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis.一种从结核分枝杆菌中分离无 DNA、纯净和完整 RNA 的稳健、高效方法。
J Microbiol Methods. 2013 Jun;93(3):198-202. doi: 10.1016/j.mimet.2013.03.018. Epub 2013 Apr 6.
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An efficient and high-yielding method for isolation of RNA from mycobacteria.一种从分枝杆菌中分离RNA的高效高产方法。
Biotechniques. 1994 Nov;17(5):834-6.
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An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.一种从难以破碎的细菌（包括分枝杆菌）中提取RNA的有效方法。
Nucleic Acids Res. 1997 Feb 1;25(3):675-6. doi: 10.1093/nar/25.3.675.
9
[Intermediate metabolism of mycobacteria. I. The cytochromes of human (H37Ra), bovine (BCG), and avian (A17) strains of Mycobacterium tuberculosis and Mycobacterium phlei].
Bull Soc Chim Biol (Paris). 1952 Mar-Apr;34(3-4):279-83.
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[Enzymatic differences in mycobacteria. Ribitol dehydrogenase].[分枝杆菌中的酶差异。核糖醇脱氢酶]
C R Acad Hebd Seances Acad Sci D. 1977 May 23;284(20):2043-5.

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Mycobacterial RNA isolation optimized for non-coding RNA: high fidelity isolation of 5S rRNA from Mycobacterium bovis BCG reveals novel post-transcriptional processing and a complete spectrum of modified ribonucleosides.针对非编码RNA优化的分枝杆菌RNA分离：从卡介苗中高保真分离5S rRNA揭示了新的转录后加工过程和完整的修饰核糖核苷谱。
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Nitrite reductase NirBD is induced and plays an important role during in vitro dormancy of Mycobacterium tuberculosis.亚硝酸盐还原酶 NirBD 在结核分枝杆菌体外休眠期间被诱导并发挥重要作用。
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