Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an, People's Republic of China.
Biosens Bioelectron. 2011 Sep 15;27(1):137-40. doi: 10.1016/j.bios.2011.06.030. Epub 2011 Jul 2.
The G-quadruplex-hemin DNAzyme has shown potential as a new catalytic label for the development of various biosensors. But the typical problem of high background induced by excess hemin itself limits the application of the method. In this work, we found that single-walled carbon nanotubes (SWNTs) can effectively decrease the background of G-quadruplex-hemin DNAzyme sensing platform. The method relies upon the strong affinity of SWNTs with hemin. In the presence of SWNTs, superfluous hemin is arrested the surface of SWNTs, and then is removed though centrifugation It was proved that SWNTs reduce the background of G-quadruplex-hemin DNAzyme sensing platform by ABTS-H(2)O(2) colorimetric. Moreover, we detected K(+) with the AGRO100 as the sensing element. The present limit of detection for K(+) is 2nM, and the sensitivity for analyzing K(+) is improved by about 10-fold as compared with the reported colorimetric counterpart in the absence of SWNTs.
四链体-血红素 DNA 酶已显示出作为开发各种生物传感器的新型催化标签的潜力。但是，过量血红素本身引起的高背景问题限制了该方法的应用。在这项工作中，我们发现单壁碳纳米管 (SWNTs) 可以有效地降低四链体-血红素 DNA 酶传感平台的背景。该方法依赖于 SWNTs 与血红素的强亲和力。在存在 SWNTs 的情况下，多余的血红素被截留到 SWNTs 的表面，然后通过离心去除。实验证明，SWNTs 通过 ABTS-H(2)O(2)比色法降低了四链体-血红素 DNA 酶传感平台的背景。此外，我们使用 AGRO100 作为传感元件来检测 K(+)。目前，K(+) 的检测限为 2nM，与没有 SWNTs 时报道的比色法相比，分析 K(+)的灵敏度提高了约 10 倍。
