自旋标记硬脂酸盐对鸭红细胞腺苷酸环化酶的影响。
Effects of spin-labeled stearates on duck erythrocyte adenylate cyclase.
作者信息
Tran P L
出版信息
Eur J Biochem. 1979 Feb 1;93(3):559-63. doi: 10.1111/j.1432-1033.1979.tb12855.x.
The effects of various spin-labeled stearates on duck erythrocyte adenylate cyclase were investigated. Only 2-(3-carboxypropyl)-4,4-dimethyl-2-tridecyl-3-oxazolidinyloxyl caused an increase in adenylate cyclase activity. It increased the basal rate by about 50%, and the activities stimulated by isoproterenol and isoproterenol plus guanosine 5'-[beta,gamma-imido]triphosphate by 35%. Upon analysis of the width parameter delta1 in the electron spin resonance spectra for both the basal enzyme activity and the stimulation obtained with effectors such as guanosine 5'-[beta,gamma-imido]triphosphate, isoproterenol, isoproterenol plus guanosine 5'-[beta,gamma-imido]triphosphate and NaF, a correlation of the changes of modification in adenylate cyclase activities was found. These findings suggest that the molecular environment of the enzyme has been modified.
研究了各种自旋标记硬脂酸盐对鸭红细胞腺苷酸环化酶的影响。只有2-(3-羧丙基)-4,4-二甲基-2-十三烷基-3-恶唑烷基氧基导致腺苷酸环化酶活性增加。它使基础速率提高了约50%，使异丙肾上腺素以及异丙肾上腺素加鸟苷5'-[β,γ-亚氨基]三磷酸刺激的活性提高了35%。在分析基础酶活性以及用鸟苷5'-[β,γ-亚氨基]三磷酸、异丙肾上腺素、异丙肾上腺素加鸟苷5'-[β,γ-亚氨基]三磷酸和NaF等效应物获得的刺激的电子自旋共振光谱中的宽度参数δ1时，发现了腺苷酸环化酶活性修饰变化之间的相关性。这些发现表明该酶的分子环境已被改变。
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