Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul, 136-701, South Korea.
Mol Biol Rep. 2012 Apr;39(4):3933-42. doi: 10.1007/s11033-011-1172-9. Epub 2011 Jul 17.
The purpose of this study was to determine the structure of the porcine PPARGC1A 5' upstream region, and to find suitable molecular markers for improved meat quality and good lean meat production. Ten DNA polymorphisms, including 7 SNPs, 2 microsatellites, and 1 insertion or deletion were newly found in the 5' upstream region of PPARGC1A. Three SNPs that had restriction enzyme site were evaluated for associations with muscle fiber characteristics and production traits. Two hundred fifty-two pigs (Yorkshire and Landrace) were used in this analysis. The c.-2894G>A genotypes was significantly associated with muscle fiber characteristics, including the number of fiber type I and IIb composition (P < 0.05), mean cross-sectional area of fibers (P < 0.01), and fiber number per unit area (P < 0.05). The animals with the GG genotype had a higher percentage of type I fibers and a lower percentage of type IIb fibers with better meat quality [higher pH value (P < 0.05) and lower drip loss (P < 0.05)] and lean meat production [larger loin eye area (P < 0.05)]. Moreover, the mRNA expression levels of PPARGC1A among genotypes were significantly different with the highest level of GG genotype. The c.-2885G>T and c.-1402A>T sites showed similar results that had significant effects on the mean cross-sectional area (CSA; P < 0.05), fiber number per unit area (P < 0.05) and loin eye area (P < 0.01). Therefore, we suggest that the c.-2894G>A polymorphism in the 5' upstream region of the porcine PPARGC1A gene can be used as a meaningful molecular marker for simultaneous improvement of lean meat production and quality traits.
本研究旨在确定猪 PPARGC1A 5'上游区域的结构，并寻找适合改善肉质和生产优质瘦肉的分子标记。在 PPARGC1A 的 5'上游区域新发现了 10 个 DNA 多态性，包括 7 个 SNP、2 个微卫星和 1 个插入或缺失。评估了 3 个具有限制性内切酶位点的 SNP 与肌肉纤维特性和生产性状的关系。本分析使用了 252 头猪（约克夏和长白）。基因型 c.-2894G>A 与肌肉纤维特性显著相关，包括纤维 I 型和 IIb 组成的数量（P < 0.05）、纤维的平均横截面积（P < 0.01）和单位面积的纤维数量（P < 0.05）。GG 基因型的动物具有更高比例的 I 型纤维和更低比例的 IIb 型纤维，具有更好的肉质[更高的 pH 值（P < 0.05）和更低的滴水损失（P < 0.05）]和更高的瘦肉生产能力[更大的腰眼面积（P < 0.05）]。此外，不同基因型之间的 PPARGC1A mRNA 表达水平存在显著差异，GG 基因型的表达水平最高。c.-2885G>T 和 c.-1402A>T 位点的结果相似，对平均横截面积（CSA；P < 0.05）、单位面积的纤维数量（P < 0.05）和腰眼面积（P < 0.01）有显著影响。因此，我们认为猪 PPARGC1A 基因 5'上游区域的 c.-2894G>A 多态性可以作为同时改善瘦肉生产和肉质性状的有意义的分子标记。
