Lippross Sebastian, Moeller Bjoern, Haas Holger, Tohidnezhad Mersedeh, Steubesand Nadine, Wruck Christoph Jan, Kurz Bodo, Seekamp Andreas, Pufe Thomas, Varoga Deike
University Medical Center Schleswig Holstein, Kiel Campus, Kiel, Germany.
Arthritis Rheum. 2011 Nov;63(11):3344-53. doi: 10.1002/art.30547.
Treatment options for rheumatoid arthritis range from symptomatic approaches to modern molecular interventions such as inhibition of inflammatory mediators. Inhibition of inflammation by platelet-rich plasma (PRP) has been proposed as a treatment for tendinitis and osteoarthritis. The present study was undertaken to investigate the effect of PRP on antigen-induced arthritis (AIA) of the knee joint in a large animal model.
Six-month-old pigs (n = 10) were systemically immunized by bovine serum albumin (BSA) injection, and arthritis was induced by intraarticular BSA injection. PRP was injected into the knee joints of 5 of the animals after 2 weeks. An additional 5 animals received no systemic immunization (controls). Signs of arthritis were documented by plain histologic analysis, Safranin O staining, and immunohistochemistry analysis for type II collagen (CII), interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF). Interleukin-1β (IL-1β), IL-6, tumor necrosis factor α (TNFα), VEGF, and insulin-like growth factor 1 (IGF-1) protein content was measured by Luminex assay.
In the pigs with AIA, plain histologic analysis revealed severe arthritic changes in the synovium. Safranin O and CII staining showed decreased proteoglycan and CII content in cartilage. Immunohistochemistry analysis revealed increased levels of IL-6 and VEGF in synovium and cartilage, and protein concentrations of IL-6, VEGF, IL-1β, and IGF-1 in synovium and cartilage were elevated as well; in addition, TNFα protein was increased in cartilage. Treatment with PRP led to attenuation of these arthritic changes in the synovium and cartilage.
We have described a porcine model of AIA. Experiments using this model demonstrated that PRP can attenuate arthritic changes as assessed histologically and based on protein synthesis of typical inflammatory mediators in the synovial membrane and cartilage.
类风湿关节炎的治疗方法从对症治疗到现代分子干预手段，如抑制炎症介质等。富血小板血浆（PRP）抑制炎症已被提议用于治疗肌腱炎和骨关节炎。本研究旨在探讨PRP对大型动物模型膝关节抗原诱导性关节炎（AIA）的影响。
对10只6月龄猪通过注射牛血清白蛋白（BSA）进行全身免疫，然后通过关节内注射BSA诱导关节炎。2周后，对其中5只动物的膝关节注射PRP。另外5只动物未进行全身免疫（作为对照）。通过普通组织学分析、番红O染色以及对II型胶原（CII）、白细胞介素-6（IL-6）和血管内皮生长因子（VEGF）进行免疫组织化学分析来记录关节炎的体征。通过Luminex检测法测量白细胞介素-1β（IL-1β）、IL-6、肿瘤坏死因子α（TNFα）、VEGF和胰岛素样生长因子1（IGF-1）的蛋白含量。
在患有AIA的猪中，普通组织学分析显示滑膜有严重的关节炎变化。番红O和CII染色显示软骨中蛋白聚糖和CII含量降低。免疫组织化学分析显示滑膜和软骨中IL-6和VEGF水平升高，滑膜和软骨中IL-6、VEGF、IL-1β和IGF-1的蛋白浓度也升高；此外，软骨中TNFα蛋白增加。PRP治疗导致滑膜和软骨中的这些关节炎变化减轻。
我们描述了一种AIA的猪模型。使用该模型进行的实验表明，PRP可减轻组织学评估以及基于滑膜和软骨中典型炎症介质蛋白合成所评估的关节炎变化。
