Bai Yan, Qin Biyin, Zhou Yanhui, Wang Yudong, Wang Zi, Zheng Wenjie
College of Life Science and Technology of Jinan University, Guangzhou 510632, People's Republics of China.
J Nanosci Nanotechnol. 2011 Jun;11(6):5012-7. doi: 10.1166/jnn.2011.4130.
This paper reported the preparation and antioxidant capacities of element selenium nanoparticles (nanoSe(0))-ascorbic acid (Vc) sol and nanSe(0)/Vc/selenocystine (SeCys) sol-gel compounds. NanoSe(0)-Vc sol was prepared by reduction of selenious dioxide (SeO2) with Vc. In the nanoSe(0)-Vc sol, highly concentrated Vc was also used as a modifier to modulate the diameter of Se(0) nanoparticles in the liquid phase. Then excellent nanoSe(0) sol-gel compounds were obtained by adding SeCys into the nanoSe(0)-Vc sol. The structure of the nanoSe(0)/Vc/SeCys sol-gel compounds was defined, which was constructed via C-Se, Se-H and O=C-Se valences and by interaction between SeCys and Vc via peptide bonds, esterification and dehydration. The antioxidant capacities of the nanoSe(0)-Vc sol and the nanoSe(0)Vc/SeCys sol-gel compounds were estimated by oxygen radical absorption capacity (ORAC) assay. The nanoSe(0)/Vc/SeCys sol-gel compounds possessed a strong antioxidant capacity due to forming the perfect three-dimensional (3D) frameworks structure. The results suggested that the nanoSe(0)-Vc sol and the nanoSe(0)Vc/SeCys sol-gel compounds might be potential medicine, especially antioxidant.
本文报道了元素硒纳米颗粒（nanoSe(0)）-抗坏血酸（Vc）溶胶以及nanSe(0)/Vc/硒代胱氨酸（SeCys）溶胶-凝胶化合物的制备及其抗氧化能力。通过用Vc还原二氧化硒（SeO2）制备了nanoSe(0)-Vc溶胶。在nanoSe(0)-Vc溶胶中，高浓度的Vc还用作改性剂来调节液相中Se(0)纳米颗粒的直径。然后通过向nanoSe(0)-Vc溶胶中加入SeCys得到了优异的nanoSe(0)溶胶-凝胶化合物。确定了nanoSe(0)/Vc/SeCys溶胶-凝胶化合物的结构，其通过C-Se、Se-H和O=C-Se价键以及SeCys与Vc之间通过肽键、酯化和脱水的相互作用构建而成。通过氧自由基吸收能力（ORAC）测定评估了nanoSe(0)-Vc溶胶和nanoSe(0)Vc/SeCys溶胶-凝胶化合物的抗氧化能力。由于形成了完美的三维（3D）框架结构，nanoSe(0)/Vc/SeCys溶胶-凝胶化合物具有很强的抗氧化能力。结果表明，nanoSe(0)-Vc溶胶和nanoSe(0)Vc/SeCys溶胶-凝胶化合物可能是潜在的药物，尤其是抗氧化剂。
