一种用于从粗样品中定量苏云金芽孢杆菌以色列亚种δ内毒素的简单溶血方法。
A simple haemolytic method for quantitation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from crude samples.
作者信息
Majumdar M K, Sikdar D P, Sarma A B, Majumdar S K
机构信息
Central Drug Laboratory, Calcutta, India.
出版信息
J Appl Bacteriol. 1990 Aug;69(2):241-6. doi: 10.1111/j.1365-2672.1990.tb01515.x.
Abstract
A simple haemolytic assay method for quantitative estimation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from a crude preparation has been developed. The method has several advantages over mosquito-larvicidal methods of assay as it is inexpensive, highly sensitive and easier to run and can be used for performing a reasonably large number of assays rapidly with high precision and with a coefficient of variation that does not exceed 1.96%.
摘要
已开发出一种简单的溶血测定方法，用于从粗制品中定量估算苏云金芽孢杆菌以色列亚种的δ内毒素。与蚊幼虫测定方法相比，该方法具有几个优点，因为它成本低、灵敏度高、操作更简便，可用于快速高精度地进行大量测定，变异系数不超过1.96%。
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