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一种编码125千道尔顿杀幼虫多肽的苏云金芽孢杆菌以色列亚种基因与反向重复序列相关。

A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted repeat sequences.

作者信息

Bourgouin C, Delécluse A, Ribier J, Klier A, Rapoport G

机构信息

Département des Biotechnologies, Institut-Pasteur, Paris, France.

出版信息

J Bacteriol. 1988 Aug;170(8):3575-83. doi: 10.1128/jb.170.8.3575-3583.1988.

DOI:10.1128/jb.170.8.3575-3583.1988
PMID:2841292
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211331/
Abstract

A gene encoding a 125-kilodalton (kDa) mosquitocidal delta-endotoxin was cloned from the 72-MDa resident plasmid of Bacillus thuringiensis subsp. israelensis. This gene is similar in its 3' region to the gene encoding the 135-kDa protein previously cloned (C. Bourgouin, A. Klier, and G. Rapoport, Mol. Gen. Genet. 205:390-397, 1986). Escherichia coli recombinant clones harboring the 125-kDa gene were toxic to larvae of the three mosquito species Aedes aegypti, Anopheles stephensi, and Culex pipiens. In addition, the B. thuringiensis subsp. israelensis DNA fragment carrying the 125-kDa protein gene contains two sets of inverted repeat sequences, identified either by the S1 nuclease method or by electron microscopic observation. The structural organization of inverted repeat sequences and of the 125-kDa gene was analyzed and suggests that this B. thuringiensis subsp. israelensis delta-endotoxin gene is located within a transposable element.

摘要

从苏云金芽孢杆菌以色列亚种的72-MDa常驻质粒中克隆出一个编码125千道尔顿（kDa）杀蚊δ-内毒素的基因。该基因的3'区域与先前克隆的编码135-kDa蛋白的基因相似（C. 布尔古安、A. 克利尔和G. 拉波波特，《分子遗传学与普通遗传学》205:390 - 397，1986年）。携带125-kDa基因的大肠杆菌重组克隆对埃及伊蚊、斯氏按蚊和致倦库蚊这三种蚊虫的幼虫具有毒性。此外，携带125-kDa蛋白基因的苏云金芽孢杆菌以色列亚种DNA片段包含两组反向重复序列，可通过S1核酸酶法或电子显微镜观察鉴定。对反向重复序列和125-kDa基因的结构组织进行了分析，结果表明该苏云金芽孢杆菌以色列亚种δ-内毒素基因位于一个转座元件内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/eea5b2385bc6/jbacter00186-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/a50ec00be38f/jbacter00186-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/f7017701baa5/jbacter00186-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/0a7ce52c8798/jbacter00186-0268-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/b07135511703/jbacter00186-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/eea5b2385bc6/jbacter00186-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/a50ec00be38f/jbacter00186-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/f7017701baa5/jbacter00186-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/0a7ce52c8798/jbacter00186-0268-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/b07135511703/jbacter00186-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/211331/eea5b2385bc6/jbacter00186-0270-a.jpg

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