Camarero V C, Junqueira V B, Colepicolo P, Karnovsky M L, Mariano M
Department of Pathology, Universidade de São Paulo, Brazil.
J Cell Physiol. 1990 Dec;145(3):481-7. doi: 10.1002/jcp.1041450313.
The release of superoxide anion (O2-) by inflammatory macrophages, multinucleated giant cells, and epithelioid cells, obtained by the insertion of round glass coverslips into the subcutaneous tissue of mice, was investigated. O2- was shown to be spontaneously released by cells on the surface of glass coverslips implanted up to 7 days, but not by cells obtained 14 or 21 days after coverslip implantation. The former showed increased O2- release when stimulated by phorbol myristate acetate, whereas cells harvested after 14 or 21 days implantation did not. The induction of delayed type hypersensitivity around coverslips implanted for 5 days increased spontaneous O2- release by these cells by 40%. On the other hand, when the same protocol was used with coverslips implanted for 14 days, O2- release was not detected. These results were viewed in regard to the composition of the cell population at each time point. When coverslips were removed after 14 days of implantation and the cells incubated for 30 minutes in vitro, the medium so conditioned inhibited O2- release by cells of 5 day old preparations. This indicates the release by cells on the longer term coverslips of a substance that inhibits O2- production by cells of coverslips implanted for 5 days only. This inhibitory activity could be suppressed by treating the conditioned medium with proteases. The factor was, however, heat stable and exerted its effects even when the test cells were exposed to phorbol myristate acetate.
通过将圆形玻璃盖玻片插入小鼠皮下组织获得炎性巨噬细胞、多核巨细胞和上皮样细胞，对其超氧阴离子（O2-）的释放进行了研究。结果表明，植入长达7天的玻璃盖玻片表面的细胞可自发释放O2-，而在盖玻片植入14天或21天后获得的细胞则不会。前者在佛波酯肉豆蔻酸酯刺激下O2-释放增加，而植入14天或21天后收获的细胞则不然。在植入5天的盖玻片周围诱导迟发型超敏反应可使这些细胞的自发O2-释放增加40%。另一方面，当对植入14天的盖玻片采用相同方案时，未检测到O2-释放。这些结果结合每个时间点的细胞群体组成进行了分析。当植入14天后取出盖玻片并将细胞在体外孵育30分钟时，如此处理的培养基可抑制5天龄制剂细胞的O2-释放。这表明长期植入盖玻片的细胞释放了一种仅抑制植入5天盖玻片细胞O2-产生的物质。用蛋白酶处理条件培养基可抑制这种抑制活性。然而，该因子对热稳定，即使受试细胞暴露于佛波酯肉豆蔻酸酯也能发挥作用。
