Polish Academy of Sciences, Warsaw, Poland.
Mol Plant Microbe Interact. 2011 Dec;24(12):1522-9. doi: 10.1094/MPMI-02-11-0025.
Antagonism of Trichoderma spp. against phytopathogenic fungi is widely exploited for biocontrol of plant diseases. A crucial role in the biocontrol mechanism is attributed to cell-wall-degrading enzymes secreted by Trichoderma spp. Therefore, more efficient production and secretion of the enzymes should elevate the biocontrol abilities of Trichoderma spp. Because the majority of secretory hydrolases are glycoproteins, it has been postulated that the posttranslational modification of these proteins could constitute a bottleneck in their production and secretion. Our previous study showed that improvement of O-glycosylation elevated protein secretion by Trichoderma reesei. In this study, we enhanced the biocontrol abilities of T. atroviride P1 against plant pathogens by overexpressing the Saccharomyces cerevisiae DPM1 gene coding for dolichyl phosphate mannose (DPM) synthase, a key enzyme in the O-glycosylation pathway. The transformants we obtained showed doubled DPM synthase activity and, at the same time, significantly elevated cellulolytic activity. They also revealed an improved antifungal activity against the plant pathogen Pythium ultimum.
木霉属拮抗植物病原菌广泛用于植物病害的生物防治。细胞壁降解酶在生物防治机制中起着关键作用，这些酶由木霉属分泌。因此，提高酶的生产和分泌效率应该能提高木霉属的生物防治能力。由于大多数分泌水解酶是糖蛋白，因此人们推测这些蛋白质的翻译后修饰可能是其生产和分泌的一个瓶颈。我们之前的研究表明，O-糖基化的改善可以提高里氏木霉的蛋白质分泌。在这项研究中，我们通过过表达酿酒酵母 DPM1 基因来增强深绿木霉 P1 对植物病原菌的生物防治能力，该基因编码参与 O-糖基化途径的关键酶——磷酸多萜醇甘露糖（DPM）合酶。我们获得的转化体的 DPM 合酶活性提高了一倍，同时纤维素酶活性也显著提高。它们还显示出对植物病原菌腐霉的抗真菌活性得到了改善。
