Watabe K, Yamada M, Kawamura T, Kim S U
Department of Medicine, University of British Columbia, Vancouver, Canada.
J Neuropathol Exp Neurol. 1990 Sep;49(5):455-67. doi: 10.1097/00005072-199009000-00001.
Cultured Schwann cells derived from adult mouse dorsal root ganglia and peripheral nerves were transfected with a plasmid containing SV-40 large T antigen gene, and 25 colonies of stable transformants were obtained, one of which was expanded and recloned. This transfected cell line, designated MS1, expressed SV-40 large T antigen and showed continuous cell growth with a doubling time of 27 hours. The MS1 cells had distinct Schwann cell phenotypes such as S-100 protein, laminin, 2',3'-cyclic nucleotide 3'-phosphodiesterase and P0 protein, as shown by immunofluorescence microscopy. When MS1 cells were exposed to dibutyryl cyclic adenosine-3',5'-monophosphate (dbc AMP), they extended long bipolar processes two- to ten-fold longer than those of untreated MS1 cells and frequently formed whorl-like alignments similar to palisade formations or organoid patterns observed in human Schwannomas and neurofibromas. These results suggest that transformed Schwann cells can be a useful model for analyzing regulatory mechanisms of Schwann cells, neuron-Schwann cell interactions and experimental Schwann cell neoplasms in vitro.
将含有SV - 40大T抗原基因的质粒转染源自成年小鼠背根神经节和周围神经的培养雪旺细胞，获得了25个稳定转化体克隆，其中一个进行扩大培养并再次克隆。这个转染的细胞系命名为MS1，表达SV - 40大T抗原，细胞持续生长，倍增时间为27小时。免疫荧光显微镜检查显示，MS1细胞具有明显的雪旺细胞表型，如S - 100蛋白、层粘连蛋白、2',3'-环核苷酸3'-磷酸二酯酶和P0蛋白。当MS1细胞暴露于二丁酰环磷腺苷（dbc AMP）时，它们伸出的双极突起比未处理的MS1细胞长2至10倍，并且经常形成类似于在人类神经鞘瘤和神经纤维瘤中观察到的栅栏状结构或类器官模式的漩涡状排列。这些结果表明，转化的雪旺细胞可以成为体外分析雪旺细胞调节机制、神经元 - 雪旺细胞相互作用和实验性雪旺细胞瘤的有用模型。
