Sobue G, Pleasure D
Science. 1984 Apr 6;224(4644):72-4. doi: 10.1126/science.6322307.
Indirect immunofluorescence was used to show the presence of galactocerebroside (galC), a lipid found in myelin, on the surface of about half of the Schwann cells isolated from neonatal rat sciatic nerves and cultured for 1 day without neurons. By day 4 in vitro, the Schwann cells had all lost their surface galC. Three days after beginning treatment with 10(-3) molar 8-bromo-adenosine 3',5'-monophosphate (8-bromo cyclic AMP) or N6,O2'-dibutyryl adenosine 3',5'-monophosphate (dibutyryl cyclic AMP), galC reappeared on the Schwann cells, and 2 days later 48 percent of the cultured Schwann cells showed surface galC. Tritium from tritiated D-galactose was incorporated into galC by the 8-bromo cyclic AMP-and dibutyryl cyclic AMP-treated cultures at a rate 15 times the control rate.
间接免疫荧光法用于显示半乳糖脑苷脂（galC）的存在，galC是一种存在于髓磷脂中的脂质，在从新生大鼠坐骨神经分离并在无神经元的情况下培养1天的约一半雪旺氏细胞表面可检测到。在体外培养第4天时，雪旺氏细胞全部失去其表面的galC。在用10⁻³摩尔的8-溴腺苷3'，5'-单磷酸（8-溴环磷酸腺苷）或N⁶，O²'-二丁酰腺苷3'，5'-单磷酸（二丁酰环磷酸腺苷）开始治疗三天后，galC重新出现在雪旺氏细胞上，2天后，48%的培养雪旺氏细胞显示有表面galC。经8-溴环磷酸腺苷和二丁酰环磷酸腺苷处理的培养物将氚化D-半乳糖中的氚掺入galC的速率是对照速率的15倍。
