Department of Pathology and Laboratory Medicine, University of Calgary and Calgary Laboratory Services, Calgary, Alberta, Canada.
BJU Int. 2012 Mar;109(5):788-95. doi: 10.1111/j.1464-410X.2011.10437.x. Epub 2011 Jul 19.
• To investigate the effects of different folic acid concentrations on the growth and invasiveness of prostate cancer cell lines. • To determine if observed changes are correlated with changes in levels of the potential prostate cancer biomarker, sarcosine, a byproduct of folate metabolism.
• The prostate cancer cell lines PC-3, LNCaP and DU145 were cultured in media containing 4, 20 or 100 nm of folic acid and assayed for growth over 9 days by counting viable cells at 3-day intervals, or for invasion by passage through a Matrigel-coated transwell membrane. • Cells grown in the different folic acid media were collected and subjected to metabolomic analysis by gas chromatography and mass spectrometry to measure levels of intracellular sarcosine.
• The results show that higher levels of folic acid can increase cell growth in PC-3 and LNCaP prostate cancer cell lines, and may also increase the invasive capacity of PC-3, LNCaP and DU145 cells. • We did not observe a correlation between increased invasion from higher folic acid concentrations and levels of sarcosine, but there were significant changes in other metabolites in cells grown in higher levels of folic acid.
• These findings suggest that folic acid has an important and potentially negative role in prostate cancer progression.
研究不同浓度叶酸对前列腺癌细胞系生长和侵袭的影响。
确定观察到的变化是否与潜在前列腺癌生物标志物琥珀酸水平的变化相关，琥珀酸是叶酸代谢的副产物。
将前列腺癌细胞系 PC-3、LNCaP 和 DU145 在含有 4、20 或 100nm 叶酸的培养基中培养，并通过每隔 3 天计数活细胞来检测 9 天内的生长情况，或通过穿过 Matrigel 涂层的 Transwell 膜进行侵袭测定。
将在不同叶酸培养基中生长的细胞收集并通过气相色谱和质谱进行代谢组学分析，以测量细胞内琥珀酸的水平。
结果表明，较高水平的叶酸可以增加 PC-3 和 LNCaP 前列腺癌细胞系的细胞生长，并且可能还增加 PC-3、LNCaP 和 DU145 细胞的侵袭能力。
我们没有观察到较高叶酸浓度下的侵袭性增加与琥珀酸水平之间的相关性，但在细胞中观察到其他代谢物的显著变化在较高叶酸水平下生长。
