使用原肌球蛋白抗体对自发转化细胞和B77超级转化细胞中的肌动蛋白细胞骨架进行免疫荧光检测。
Immunofluorescent detection of actin cytoskeleton in spontaneously transformed and B77-supertransformed cells using antibody to tropomyosin.
作者信息
Urbancíková M, Grófová M
机构信息
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
出版信息
Neoplasma. 1990;37(6):703-10.
Differences in immunofluorescence of actin cytoskeleton between normal rat fibroblasts and two transformed cell lines SAMIV and SAMB77 were detected by antiserum to tropomyosin. In both transformed cell lines reduction in number and shortening of microfilament bundles (stress fibers) were observed. In some transformed cells ruffling membranes (peripheral ruffles) were seen. Differences were found in actin cytoskeleton among individual cells of spontaneous transformants (SAMIV) and supertransformants (SAMB77).
用抗原肌球蛋白抗血清检测正常大鼠成纤维细胞与两种转化细胞系SAMIV和SAMB77之间肌动蛋白细胞骨架的免疫荧光差异。在两种转化细胞系中均观察到微丝束（应力纤维）数量减少和缩短。在一些转化细胞中可见膜波动（周边波动）。在自发转化细胞（SAMIV）和超转化细胞（SAMB77）的单个细胞之间的肌动蛋白细胞骨架中发现了差异。
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