Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Comp Biochem Physiol C Toxicol Pharmacol. 2011 Nov;154(4):318-25. doi: 10.1016/j.cbpc.2011.06.019. Epub 2011 Jul 13.
A combination of proteomic and biochemical assays was used to examine variations in the venom of Conus vexillum taken from two locations (Hurgada and Sharm El-Shaikh) in the Red Sea, Egypt. Using MALDI/TOF-MS, a remarkable degree of intra-species variation between venom samples from both locations was identified. To evaluate variability in the cytotoxic effects of Conus venom, mice were injected with the same dose from each location. The oxidative stress biomarkers [malondialdehyde (MDA), protein carbonyl content (PCC)], antioxidants [glutathione (GSH), superoxide dismutase (SOD), catalase (CAT)], total antioxidant capacity (TAC) and nitric oxide (NO), were measured 3, 6, 9 and 12h post venom injection. The venoms induced a significant increase in the levels of PCC, MDA, NO, GSH and CAT. The venoms significantly inhibited the activity of SOD and reduced the TAC. Toxicological data showed that the venom obtained from Hurgada was more potent than that obtained from Sharm El-Shaikh. It can be concluded that: (1) the venom of the same Conus species from different regions is highly diversified (2) the venoms from different locations reflect clear differences in venom potency and (3) the cytotoxic effects of C. vexillum venom can be attributed to its ability to induce oxidative stress.
采用蛋白质组学和生化分析方法，研究了来自埃及红海两个地区（赫尔格达和沙姆沙伊赫）的 Conus vexillum 毒液的变化。利用 MALDI/TOF-MS，鉴定出来自两个地区的毒液样本之间存在显著的种内变异。为了评估 Conus 毒液的细胞毒性作用的变异性，用相同剂量的毒液对小鼠进行注射。注射毒液 3、6、9 和 12 小时后，测量氧化应激生物标志物[丙二醛 (MDA)、蛋白羰基含量 (PCC)]、抗氧化剂[谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)]、总抗氧化能力 (TAC) 和一氧化氮 (NO)。毒液导致 PCC、MDA、NO、GSH 和 CAT 的水平显著增加。毒液显著抑制 SOD 的活性并降低 TAC。毒理学数据表明，来自赫尔格达的毒液比来自沙姆沙伊赫的毒液更有效。可以得出结论：(1) 来自不同地区的相同 Conus 物种的毒液高度多样化；(2) 来自不同地区的毒液反映了毒液效力的明显差异；(3) C. vexillum 毒液的细胞毒性作用可归因于其诱导氧化应激的能力。
