Department of Parasitology and Microbiology, School of Medicine, Nantong University, Nantong, Jiangsu Province, 226001, China.
Asian Pac J Trop Med. 2011 May;4(5):353-6. doi: 10.1016/S1995-7645(11)60102-8. Epub 2011 Jun 22.
To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii (P. carinii) against P. carinii in mice.
The fragment of the antigen within p55(p55-582) was cloned. Then recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1(+). BALB/c mice were used as experimental models to examine the immunogenicity of pcDNA3.1(+)-p55-582. ELISA and RT-PCR were used to evaluate the role of this kind of DNA vaccine.
The results of western blot indicated that the recombinant DNA[pcDNA3.1(+)-p55-582] could be expressed correctly and had antigenicity in transfected COS-7 cells. ELISA and RT-PCR showed that pcDNA3.1(+)-p55-582 elicited antibody production, stimulated lymphocyte proliferation and provided partial protection by reducing the P. carinii burden.
The data demonstrate that pcDNA3.1(+)-p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.
评估编码肺孢子菌 55kDa 抗原片段（P. carinii）基因的 DNA 疫苗对小鼠肺孢子菌的保护作用。
克隆抗原内的片段 p55(p55-582)。然后基于真核表达载体 pcDNA3.1(+)构建重组质粒。BALB/c 小鼠作为实验模型，检测 pcDNA3.1(+)-p55-582 的免疫原性。ELISA 和 RT-PCR 用于评估这种 DNA 疫苗的作用。
Western blot 结果表明，重组 DNA[pcDNA3.1(+)-p55-582]可在转染的 COS-7 细胞中正确表达且具有抗原性。ELISA 和 RT-PCR 显示 pcDNA3.1(+)-p55-582 可诱导抗体产生、刺激淋巴细胞增殖，并通过减轻肺孢子菌负担提供部分保护。
数据表明，pcDNA3.1(+)-p55-582 可能是一种有效的疫苗，可为免疫动物提供部分保护。
