海洋盐生植物鹿根树皮提取物对 CCL(4)诱导肝毒性的保肝和抗氧化作用。
Hepatoprotective and antioxidant properties of marine halophyte Luminetzera racemosa bark extract in CCL(4) induced hepatotoxicity.
机构信息
School of Marine Sciences, Department of Oceanography and Coastal Area Studies, Alagappa University, Thondi Campus, Thondi 623409, Tamilnadu, India.
出版信息
Asian Pac J Trop Med. 2011 Jun;4(6):462-5. doi: 10.1016/S1995-7645(11)60126-0.
OBJECTIVE
To identify the hepatoprotective and antioxidant activity of Luminetzera racemosa (L. racemosa) bark extract.
METHODS
Wistar albino rats were divided into 6 groups: Group 1 served as control; Group 2 served as hepatotoxin (CCL(4) treated) group; Group 3 served as positive control (Silymarin) treated groups; Group 4, 5 and 6 served as (100, 200 and 300 mg/kg bw p.o.) L. racemosa bark extract treated groups. Moreover, in vitro antioxidant indexes, including DPPH, hydroxyl radical scavenging activity (HRSA), NO, ferric reducing antioxidant power (FRAP), lipid hydroperoxide (LPO) and super oxide dismutase (SOD) were also analyzed in the bark extract.
RESULTS
The results suggested that, the level of serum glutamate oxyloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatise (ALP), bilurubin, cholesterol, sugar and lactate dehydrogenase (LDH) were significantly (P<0.05) increased in hepatotoxin treated rats when compared with the control group. But, the maximum reduction of SGOT [(225.36±13.65) IU/L], SGPT [(96.85±17.36) IU/L], ALP [(315.37±17.16) IU/L], bilirubin [(2.97±0.46) mg/dL], cholesterol [(163.73±17.54) mg/dL], sugar [(127.35±27.35) mg/dL] and LDH [(1 784.00±268.36) IU/L] were observed with 300 mg/kg bw of bark extract treated rats. Histopathological scores showed that, no visible changes were observed with high dose (300 mg/kg bw) of bark extract treated rats except mild fatty changes. The in vitro antioxidant assays showed that, the IC(50) values were observed as (44.17±6.87) μg/mL, (42.45±2.81)μg/mL, (62.37±3.98)μg/mL, (54.24±3.09)μg/mL, (87.25±5.90) μg/mL and (71.54±5.42)μg/mL for DPPH, HRSA, NO, FRAP, LPO and SOD radical scavenging activities, respectively.
CONCLUSIONS
The hepatoprotective and antioxidant activities of the bark extract might be to the presence of unique chemical classes such as flavonoids, alkaloids and polyphenols.
目的
鉴定露兜树（L. racemosa）树皮提取物的保肝和抗氧化活性。
方法
将 Wistar 白化大鼠分为 6 组：第 1 组为对照组；第 2 组为肝毒素（CCL（4）处理）组；第 3 组为阳性对照组（水飞蓟素）处理组；第 4、5 和 6 组为（100、200 和 300mg/kg bw p.o.）露兜树树皮提取物处理组。此外，还分析了树皮提取物的体外抗氧化指标，包括 DPPH、羟基自由基清除活性（HRSA）、NO、铁还原抗氧化能力（FRAP）、脂质过氧化物（LPO）和超氧化物歧化酶（SOD）。
结果
结果表明，与对照组相比，肝毒素处理大鼠的血清谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）、碱性磷酸酶（ALP）、胆红素、胆固醇、糖和乳酸脱氢酶（LDH）水平显著升高（P<0.05）。但是，SGOT[（225.36±13.65）IU/L]、SGPT[（96.85±17.36）IU/L]、ALP[（315.37±17.16）IU/L]、胆红素[（2.97±0.46）mg/dL]、胆固醇[（163.73±17.54）mg/dL]、糖[（127.35±27.35）mg/dL]和 LDH[（1784.00±268.36）IU/L]的最大降低均观察到 300mg/kg bw 树皮提取物处理大鼠。组织病理学评分显示，除轻度脂肪变性外，高剂量（300mg/kg bw）树皮提取物处理大鼠未见明显变化。体外抗氧化试验表明，DPPH、HRSA、NO、FRAP、LPO 和 SOD 自由基清除活性的 IC（50）值分别为（44.17±6.87）μg/mL、（42.45±2.81）μg/mL、（62.37±3.98）μg/mL、（54.24±3.09）μg/mL、（87.25±5.90）μg/mL 和（71.54±5.42）μg/mL。
结论
树皮提取物的保肝和抗氧化活性可能归因于其独特的化学类群，如类黄酮、生物碱和多酚。
Zotero 插件