Zarezade Vahid, Moludi Jalal, Mostafazadeh Mostafa, Mohammadi Mohammad, Veisi Ali
Behbahan Faculty of Medical Sciences, Behbahan, Iran.
Nutrition Research Center, Department of Biochemistry and Diet Therapy, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
Avicenna J Phytomed. 2018 Jan-Feb;8(1):51-62.
The present study was conducted to investigate the antioxidant and hepatoprotective activity of the hydro-alcoholic extract of aerial parts of (HAAD) against CCl-induced hepatotoxicity in rats.
The antioxidant activity was evaluated by reducing power, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Rats were pre-treated with either 50, 100, and 200 mg/kg of HAAD or silymarin (100 mg/kg; served as the positive control group) for 15 days and they received a single dose of CCl on the last day. Hepatoprotective effects were investigated by assessment of serum biochemical enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB), malondialdehyde (MDA), and antioxidant enzymes (SOD, CAT, GST and GSH), along with histopathological studies.
Total phenolic content was 197.22±3.73 mg gallic acid equivalent/g HAAD dry weight. HAAD indicated powerful activity in FRAP, DPPH and ABTS tests. Acute toxicity study showed that the extract had an LD of >5000 mg/kg. Oral treatment with HAAD exhibited a significant decrease in the levels of AST, ALT, ALP and TB and an increase in the level of TP. The extract significantly diminished MDA levels. The activities of the antioxidant enzymes were significantly augmented in rats pretreated with HAAD 200 mg/kg. Histopathological examination demonstrated lower liver damage in HAAD-treated groups as compared to CCl groups.
Our findings indicated hepatoprotective effects of the hydro-alcoholic extract of on CCl-induced hepatic damage in rats and suggested that these effects may be produced by reducing oxidative stress.
本研究旨在探讨[植物名称]地上部分水醇提取物（HAAD）对四氯化碳诱导的大鼠肝毒性的抗氧化和保肝活性。
通过还原能力、2,2-二苯基-1-苦基肼（DPPH）和2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）试验评估抗氧化活性。大鼠分别用50、100和200mg/kg的HAAD或水飞蓟宾（100mg/kg；作为阳性对照组）预处理15天，并在最后一天接受单次剂量的四氯化碳。通过评估血清生化酶如丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、总蛋白（TP）、总胆红素（TB）、丙二醛（MDA）和抗氧化酶（SOD、CAT、GST和GSH）以及组织病理学研究来研究保肝作用。
总酚含量为197.22±3.73mg没食子酸当量/g HAAD干重。HAAD在FRAP、DPPH和ABTS试验中显示出强大的活性。急性毒性研究表明该提取物的半数致死量>5000mg/kg。口服HAAD治疗可使AST、ALT、ALP和TB水平显著降低，TP水平升高。该提取物显著降低了MDA水平。在预处理200mg/kg HAAD的大鼠中，抗氧化酶的活性显著增强。组织病理学检查表明，与四氯化碳组相比，HAAD治疗组的肝损伤较轻。
我们的研究结果表明[植物名称]地上部分水醇提取物对四氯化碳诱导的大鼠肝损伤具有保肝作用，并表明这些作用可能是通过降低氧化应激产生的。
