Omrani Mir Davood, Bazargani Soroush, Bagheri Morteza, Yazdan-Nejad Hamed
Department of Genetics, Urmia University of Medical Sciences, Urmia, Iran.
J Res Med Sci. 2009 Jul;14(4):217-22.
A single nucleotide variation within catechol-o-methyl transferase (COMT) gene may alter the COMT enzyme activity level. Polymorphism of Val158Met in the COMT gene has been related to malignancy. In this regard, a study was carried out to find a possible association between the COMT gene polymorphism in patients with sporadic prostate cancer (PCa) and benign prostatic hyperplasia (BPH).
All types of COMT158 Val/Met polymorphism were carried out using ASO-PCR method in 41 patients with prostate cancer, 193 patients with benign prostatic hyperplasia and 107 healthy male individuals.
The results of this study showed that the frequency of low producer allele A at codon 158 of the COMT gene is significantly different in BPH group compared to normal male control group (OR, 95% CI, p value 1.95: 1.46, 2.44, 0.021, respectively). However no significant difference was noticed when the comparison was made between prostate cancer group and normal male control group and also between BPH and PCa groups.
Decreased level of catechol-o-methyl transferase gene activity may play a possible role in benign prostatic hyperplasia development but not in prostate cancer. Increased level of COMT gene activity has a protective role against BPH.
儿茶酚-O-甲基转移酶（COMT）基因内的单核苷酸变异可能会改变COMT酶的活性水平。COMT基因中Val158Met的多态性与恶性肿瘤有关。在这方面，开展了一项研究以探寻散发性前列腺癌（PCa）患者和良性前列腺增生（BPH）患者中COMT基因多态性之间可能存在的关联。
采用等位基因特异性PCR（ASO-PCR）方法对41例前列腺癌患者、193例良性前列腺增生患者和107名健康男性个体进行了所有类型的COMT158 Val/Met多态性检测。
本研究结果显示，与正常男性对照组相比，COMT基因第158位密码子低活性等位基因A在BPH组中的频率有显著差异（OR值、95%置信区间、p值分别为1.95: 1.46, 2.44, 0.021）。然而，前列腺癌组与正常男性对照组之间以及BPH组与PCa组之间进行比较时，未发现显著差异。
儿茶酚-O-甲基转移酶基因活性水平降低可能在良性前列腺增生的发生中起作用，但在前列腺癌中并非如此。COMT基因活性水平升高对BPH具有保护作用。
