牛晶状体上皮细胞中的胰岛素和胰岛素样生长因子-I受体。

Receptors for insulin and IGF-I in bovine lens epithelium.

作者信息

Sinha A C, Stuart C A, Srivastava S K

机构信息

Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston 77550.

出版信息

Lens Eye Toxic Res. 1990;7(2):133-41.

PMID:2177352

Abstract

The presence of insulin receptors has not yet been established in lens epithelial cells. Our goal was to understand the involvement of insulin in lens pathology, especially diabetic cataractogenesis. As a first step in this process, we evaluated the presence of insulin receptors in bovine lens epithelium. Insulin bound to insulin receptor with a high affinity (KD 7.0 nM), although this binding of insulin to its receptor could be replaced by IGF-I (KD 41 nM). Similarly IGF-I binding to IGF-I receptor (KD 0.38 nM) could be inhibited by insulin (KD 53.7 nM). SDS-PAGE and subsequent autoradiography of affinity labeled insulin receptor show the presence of a 130 kDa band, indicating that the lens epithelium insulin receptor may be similar to insulin receptors isolated from other tissues.

摘要

晶状体上皮细胞中尚未证实胰岛素受体的存在。我们的目标是了解胰岛素在晶状体病理学，尤其是糖尿病性白内障形成过程中的作用。作为这一过程的第一步，我们评估了牛晶状体上皮细胞中胰岛素受体的存在情况。胰岛素以高亲和力（解离常数KD为7.0 nM）与胰岛素受体结合，尽管胰岛素与其受体的这种结合可被IGF-I（解离常数KD为41 nM）取代。同样，胰岛素（解离常数KD为53.7 nM）可抑制IGF-I与IGF-I受体的结合（解离常数KD为0.38 nM）。SDS-PAGE及随后对亲和标记的胰岛素受体进行的放射自显影显示存在一条130 kDa的条带，表明晶状体上皮胰岛素受体可能与从其他组织分离的胰岛素受体相似。