Department of Biology, Faculty of Science, University of Isfahan, Isfahan 81746-73696, Iran.
Appl Biochem Biotechnol. 2011 Oct;165(3-4):1068-74. doi: 10.1007/s12010-011-9325-9. Epub 2011 Jul 20.
Various chemical surfactants could affect permeability of yeast cells. In this study, effects of the surfactant addition upon yeast cells permeability and citric acid (CA) production by Yarrowia lipolytica strains DSM 3286 and M7 were investigated. The addition of Triton X-100 increased 1.4-1.8-fold of the maximum CA quantity achieved for both strains, with final CA concentrations ranging between 75-85 g/l that correspond to CA conversion yields per unit of glucose consumed of ~0.80-0.84 g/g. Scanning electron micrographs of yeast cells showed that the cells treated with Triton X-100 had altered cell structure and were smaller and narrower compared with the non-treated ones. The results showed that Triton X-100 could be used in order to increase the efficiency of CA production by Y. lipolytica strains.
各种化学表面活性剂都会影响酵母细胞的通透性。在这项研究中，考察了添加表面活性剂对解脂耶氏酵母（Yarrowia lipolytica）DSM 3286 和 M7 菌株通透性以及柠檬酸（CA）产量的影响。添加 Triton X-100 可使两种菌株的最大 CA 产量分别增加 1.4-1.8 倍，最终 CA 浓度在 75-85g/L 之间，这相当于单位葡萄糖消耗的 CA 转化率约为 0.80-0.84g/g。扫描电子显微镜观察显示，用 Triton X-100 处理的酵母细胞结构发生了改变，与未经处理的细胞相比，细胞变小变窄。结果表明，Triton X-100 可用于提高解脂耶氏酵母菌株 CA 产量的效率。
