ADP/ATP载体导入线粒体：两种受体并行发挥作用。
Import of ADP/ATP carrier into mitochondria: two receptors act in parallel.
作者信息
Steger H F, Söllner T, Kiebler M, Dietmeier K A, Pfaller R, Trülzsch K S, Tropschug M, Neupert W, Pfanner N
机构信息
Institut für Physiologische Chemie, Universität München, Federal Republic of Germany.
出版信息
J Cell Biol. 1990 Dec;111(6 Pt 1):2353-63. doi: 10.1083/jcb.111.6.2353.
Abstract
We have identified the yeast homologue of Neurospora crassa MOM72, the mitochondrial import receptor for the ADP/ATP carrier (AAC), by functional studies and by cDNA sequencing. Mitochondria of a yeast mutant in which the gene for MOM72 was disrupted were impaired in specific binding and import of AAC. Unexpectedly, we found a residual, yet significant import of AAC into mitochondria lacking MOM72 that occurred via the receptor MOM19. We conclude that both MOM72 and MOM19 can direct AAC into mitochondria, albeit with different efficiency. Moreover, the precursor of MOM72 apparently does not require a positively charged sequence at the extreme amino terminus for targeting to mitochondria.
摘要
通过功能研究和cDNA测序，我们已鉴定出粗糙脉孢菌MOM72（ADP/ATP载体（AAC）的线粒体输入受体）的酵母同源物。在一个酵母突变体中，MOM72基因被破坏，其线粒体在AAC的特异性结合和输入方面存在缺陷。出乎意料的是，我们发现，通过受体MOM19，AAC仍能大量输入缺乏MOM72的线粒体中。我们得出结论，MOM72和MOM19都能将AAC导入线粒体，尽管效率不同。此外，MOM72的前体显然不需要在极端氨基末端有带正电荷的序列来靶向线粒体。
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