Morland J, Olsen H
Drug Metab Dispos. 1977 Nov-Dec;5(6):511-7.
Suspensions of isolated liver cells were prepared from rat livers perfused with Ca++-free buffer and 0.05% collagenase. Primary cell suspensions (containing both parenchymal and nonparenchymal liver cells) metabolized sulfadimidine, sulfanilamide, p-aminobenzoic acid, and isoniazid approximately at first order kinetics for at least 4 hr. Suspensions of parenchymal cells had the same metabolic capacity, although the metabolism of isoniazid proceeded at a somewhat reduced rate compared to the primary cell suspensions. Suspensions of nonparenchymal cells did not metabolize sulfadimidine, sulfanilamide, or p-aminobenzoic acid during 4 hr, although such suspensions acted upon isoniazid to some degree. It was concluded that parenchymal rat liver cells may metabolize (acetylate) all four drugs tested, whereas nonparenchymal cells metabolize only isoniazid to any considerable extent.
从用无钙缓冲液和0.05%胶原酶灌注的大鼠肝脏中制备分离的肝细胞悬液。原代细胞悬液（包含实质肝细胞和非实质肝细胞）代谢磺胺二甲嘧啶、磺胺、对氨基苯甲酸和异烟肼，至少4小时内近似按一级动力学进行。实质肝细胞悬液具有相同的代谢能力，尽管与原代细胞悬液相比，异烟肼的代谢速率有所降低。非实质肝细胞悬液在4小时内不代谢磺胺二甲嘧啶、磺胺或对氨基苯甲酸，尽管这类悬液对异烟肼有一定作用。得出的结论是，大鼠肝脏实质细胞可能代谢（乙酰化）所测试的所有四种药物，而非实质细胞仅在相当程度上代谢异烟肼。
