Wang H W
Department of Otolaryngology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Eur Arch Otorhinolaryngol. 1990;247(6):361-3. doi: 10.1007/BF00179007.
The alpha-2 adrenoceptor radioligand tritiated yohimbine was used to determine the location of pre- versus postsynaptic alpha-2 adrenoceptors in canine nasal mucosa. Using Scatchard analysis, the maximal binding capacity (Bmax) and an apparent equilibrium dissociation constant (Kd) of the alpha-2 adrenoceptor were measured in seven normal dogs and in seven dogs subjected to a unilateral superior cervical ganglionectomy. The results showed that most alpha-2 adrenoceptors in the canine nasal mucosa were located at postsynaptic membranes and very few were located at presynaptic membranes. After superior cervical ganglionectomy, there were no significant changes of the affinity (Kd) and number (Bmax) of the alpha-2 adrenoceptors in the mucosa. These results suggest that the postsynaptic alpha-2 receptors are located extrasynaptically.
使用α-2肾上腺素能受体放射性配体氚化育亨宾来确定犬鼻黏膜中突触前与突触后α-2肾上腺素能受体的位置。采用Scatchard分析，在7只正常犬和7只接受单侧颈上神经节切除术的犬中测量了α-2肾上腺素能受体的最大结合容量（Bmax）和表观平衡解离常数（Kd）。结果表明，犬鼻黏膜中的大多数α-2肾上腺素能受体位于突触后膜，而位于突触前膜的很少。颈上神经节切除术后，黏膜中α-2肾上腺素能受体的亲和力（Kd）和数量（Bmax）无显著变化。这些结果表明，突触后α-2受体位于突触外。
