Majeed Muhammad Mudassar, Bukhari Mulazim Hussain
Department of Pathology, King Edward Medical University, 26 MOF, GOR-3, Shahdman-Lahore 54000, Pakistan.
Patholog Res Int. 2011;2011:851524. doi: 10.4061/2011/851524. Epub 2011 Jun 30.
Background. Tuberculosis is the commonest infectious disease in the developing world. Many diagnostic tests are devised for its detection including direct smear examination. This study was designed to determine the frequency of cases positive for AFB and positive for fungus in patients diagnosed to have granulomatous inflammation on Fine Needle Aspiration Cytology using special stains. Materials and Methods. A descriptive cross-sectional survey was done on 100 cases of granulomatous inflammation consistent with tuberculosis diagnosed on fine needle aspiration cytology at the Department of Pathology, King Edward Medical University, Lahore. After reporting granulomatous inflammation on Hematoxylin & Eosin staining of aspirates from FNAC, some unstained slides were subjected to special stains, like ZN, GMS, and PAS. Cases positive for AFB on ZN stain and fungus on GMS/PAS were noted down along with their frequency and percentages. Results. Forty-four cases (44%) of AFB positive smears were reported in granulomatous inflammation while only 5% cases of fungus were reported down. Cervical lymph nodes were the most commonly involved site (87%), and females were affected more (62%) than males. Most cases of AFB-positive smears were associated with caseation necrosis (93%). Conclusion. Special stains should be done on all granulomatous inflammation cases seen on FNAC for confirmation of TB and ruling out other infectious causes.
背景。结核病是发展中国家最常见的传染病。为其检测设计了许多诊断测试，包括直接涂片检查。本研究旨在通过特殊染色确定在细针穿刺细胞学检查中诊断为肉芽肿性炎症的患者中抗酸杆菌阳性和真菌阳性病例的频率。材料与方法。对拉合尔爱德华国王医科大学病理科100例经细针穿刺细胞学检查诊断为符合结核病的肉芽肿性炎症病例进行了描述性横断面调查。在对细针穿刺抽吸物进行苏木精和伊红染色报告肉芽肿性炎症后，一些未染色的玻片进行了特殊染色，如齐-尼氏染色、吉姆萨染色和过碘酸雪夫染色。记录齐-尼氏染色抗酸杆菌阳性和吉姆萨染色/过碘酸雪夫染色真菌阳性的病例及其频率和百分比。结果。在肉芽肿性炎症中报告了44例（44%）抗酸杆菌涂片阳性病例，而仅报告了5%的真菌病例。颈部淋巴结是最常受累的部位（87%），女性受累（62%）多于男性。大多数抗酸杆菌涂片阳性病例与干酪样坏死相关（93%）。结论。对于细针穿刺细胞学检查中发现的所有肉芽肿性炎症病例，均应进行特殊染色，以确诊结核病并排除其他感染原因。
