Institute of Experimental Medicine and Biology of Cuyo (IMBECU), National Council of Research (CONICET), Mendoza, Argentina.
Food Funct. 2010 Oct;1(1):124-9. doi: 10.1039/c0fo00077a. Epub 2010 Sep 22.
The present study examines the effect of chronic administration of dealcoholized red wine Malbec (DRW) on vascular remodeling and NAD(P)H oxidase and endothelial nitric oxide synthase activity (eNOS) in an experimental model of metabolic syndrome induced by fructose administration. Thirty-day old male Wistar rats were fed a normal rat diet (control) or the same diet plus 10% fructose in drinking water (FFR). During the last 4 weeks of a 10-week period of the corresponding diet, a subgroup of control and FFR (n=8 each) received DRW in their drinking water. Systolic blood pressure (SBP), a homeostasis model assessment of insulin resistance (HOMA-IR), aortic NAD(P)H oxidase and eNOS activity in the heart and vascular tissue were evaluated. Vascular remodeling was evaluated in the left carotid artery (CA) and interlobar, arcuate and interlobular renal arteries (RA) through lumen to media (L/M) ratio determination. At the end of the study FFR increased the SBP (p < 0.001), HOMA-IR (p < 0.001), and aortic NAD(P)H oxidase activity (p < 0,05) but reduced cardiac and vascular eNOS activity (p < 0.01), L/M ratio in CA (p < 0.001) and RA (p < 0.01) compared with the C group. DRW reduced SBP (p < 0.05), aortic NAD(P)H oxidase (p < 0.05), and recovered eNOS activity (p < 0.001) and L/M in CA (p < 0.001) and RA (p < 0.001) compared with FFR. This study provides new data about the beneficial effect of DRW on oxidative stress and vascular remodeling in the experimental model of metabolic syndrome. Data suggest the participation of mechanisms involving oxidative stress in FFR alterations and the usefulness of natural antioxidant substances present in red wine in the reversion of these changes.
本研究探讨了慢性给予脱醇马尔贝克红酒（DRW）对果糖诱导代谢综合征模型中血管重构以及 NAD（P）H 氧化酶和内皮型一氧化氮合酶活性（eNOS）的影响。30 日龄雄性 Wistar 大鼠给予正常大鼠饮食（对照组）或相同饮食加 10%果糖饮用水（FFR）。在 10 周相应饮食的最后 4 周期间，对照组和 FFR 的一个亚组（每组 8 只）给予 DRW 饮用水。评估收缩压（SBP）、胰岛素抵抗的稳态模型评估（HOMA-IR）、心脏和血管组织中的主动脉 NAD（P）H 氧化酶和 eNOS 活性。通过管腔到中膜比（L/M）比值的测定，评估左颈动脉（CA）和叶间、弓形和小叶间肾动脉（RA）的血管重构。研究结束时，FFR 增加了 SBP（p < 0.001）、HOMA-IR（p < 0.001）和主动脉 NAD（P）H 氧化酶活性（p < 0.05），但降低了心脏和血管 eNOS 活性（p < 0.01），L/M 比值在 CA（p < 0.001）和 RA（p < 0.01）。与 C 组相比，DRW 降低了 SBP（p < 0.05）、主动脉 NAD（P）H 氧化酶（p < 0.05），并恢复了 CA（p < 0.001）和 RA（p < 0.001）的 eNOS 活性和 L/M。本研究提供了关于 DRW 在代谢综合征实验模型中对氧化应激和血管重构的有益作用的新数据。数据表明，氧化应激参与了 FFR 改变，而红酒中存在的天然抗氧化物质在逆转这些变化方面是有用的。
