Department of Chemistry and Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
Integr Biol (Camb). 2011 Aug;3(8):816-22. doi: 10.1039/c1ib00009h. Epub 2011 Jul 21.
The binding of a ligand to a cell-surface receptor represents a first step in transmitting extracellular information to the cell in order to activate downstream intracellular signaling events. This report describes the de novo engineering of cells with a transmembrane signal transduction motif that is developed from first principles. The signaling event is initiated with a polyvalent ligand that clusters an engineered receptor in the membrane. The receptor presents a cytosolic kinase substrate that undergoes autocatalytic phosphorylation when organized in a cluster, but not in the unclustered arrangement. The example described here may represent a motif that can be used in synthetic biology for the engineering of cells with novel circuits.
配体与细胞表面受体的结合代表了将细胞外信息传递到细胞内以激活下游细胞内信号事件的第一步。本报告描述了从头开始设计具有跨膜信号转导基序的细胞，该基序是从第一性原理发展而来的。信号事件由多价配体引发，该配体在膜中聚集工程受体。该受体呈现细胞溶质激酶底物，当在簇中组织时会经历自动催化磷酸化，但在未聚集的排列中不会。这里描述的例子可能代表一种可以在合成生物学中用于工程具有新型电路的细胞的基序。
