[弓形虫在Vero细胞培养中的产生]
[Production of Toxoplasma gondii in Vero cell culture].
作者信息
Sivrikaya Gizem, Bağrıaçık Emin Ümit
机构信息
Gazi Üniversitesi Tıp Fakültesi, İmmünoloji Araştırma ve Uygulama Merkezi, Ankara, Türkiye, Turkey.
出版信息
Turkiye Parazitol Derg. 2011;35(2):61-4. doi: 10.5152/tpd.2011.16.
OBJECTIVE
To investigate parasite-host dynamics in cultures of Toxoplasma gondii (T. gondii).
METHODS
T. gondii tachyzoites were incubated in Vero-E6 cell cultures at 37°C, 5％CO₂. Tachyzoites and host cells were characterized by light microscopy. Growth kinetics of the parasite were determined.
RESULTS
Doubling time of tachyzoites and viability of host cells were determined by comparing tachyzoite cultures and control Vero cell cultures that were free of parasites. Tachyzoites harvested from cell cultures were established as a line for future studies. Purified tachyzoit line was named as GPK-001, a catalog name for the line.
CONCLUSION
In this study, we showed that an intracellular parasite, T. gondii can be produced in cell cultures under sterile conditions. We believe that the tachyzoite line established in this study would be useful in many other studies and provide answers to questions regarding biology and treatment of T. gondii infections.
目的
研究刚地弓形虫培养物中的寄生虫-宿主动态。
方法
将刚地弓形虫速殖子在37°C、5％二氧化碳条件下于Vero-E6细胞培养物中孵育。通过光学显微镜对速殖子和宿主细胞进行鉴定。测定寄生虫的生长动力学。
结果
通过比较速殖子培养物和无寄生虫的对照Vero细胞培养物，确定速殖子的倍增时间和宿主细胞的活力。从细胞培养物中收获的速殖子被建立为用于未来研究的细胞系。纯化的速殖子细胞系被命名为GPK-001，这是该细胞系的目录名称。
结论
在本研究中，我们表明细胞内寄生虫刚地弓形虫可在无菌条件下的细胞培养物中产生。我们相信本研究中建立的速殖子细胞系将在许多其他研究中有用，并为有关刚地弓形虫感染的生物学和治疗问题提供答案。
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