Bubnov N V, Basnak'ian A G, Botrin I I
Biull Eksp Biol Med. 1990 Oct;110(10):370-2.
Comparison of catalytic properties of a Mn2(+)-dependent and a Ca2+, Mg2+ dependent endonucleases of rat liver cell nuclei was carried out. The Mn2(+)-dependent endonuclease has Mr 31 kDa by SDS-PAAG-electrophoresis; pH optimum 5.5; calcium-magnesium synergism less than 3 in rat liver DNA, RF M13 DNA and phage M13 DNA. The rate of hydrolysis of single strand and double strand circular DNA was the same. The Mn2(+)-dependent endonuclease split DNA by double hit manner, and didn't change the manner in the presence of different divalent cations. Ca2+, Mg2(+)-dependent endonuclease has pH optimum 6.5; calcium-magnesium synergism up to 40 in rat liver DNA and 175 in RF M13 DNA. The rate of hydrolysis of single strand DNA was higher than double-strand DNA.
对大鼠肝细胞核中一种依赖Mn2+的核酸内切酶和一种依赖Ca2+、Mg2+的核酸内切酶的催化特性进行了比较。通过SDS-PAAG电泳，依赖Mn2+的核酸内切酶的分子量为31 kDa；最适pH为5.5；在大鼠肝DNA、RF M13 DNA和噬菌体M13 DNA中，钙镁协同作用小于3。单链和双链环状DNA的水解速率相同。依赖Mn2+的核酸内切酶以双击方式切割DNA，在不同二价阳离子存在下方式不变。依赖Ca2+、Mg2+的核酸内切酶最适pH为6.5；在大鼠肝DNA中钙镁协同作用高达40，在RF M13 DNA中高达175。单链DNA的水解速率高于双链DNA。
